RT Journal Article
T1 Identification of tumor-associated antigens with diagnostic ability of colorectal cancer by in-depth immunomic and seroproteomic analysis
A1 Garranzo Asensio, María
A1 San Segundo Acosta, Pablo
A1 Povés Francés, Carmen
A1 Fernández Aceñero, María Jesús
A1 Martínez Useros, Javier
A1 Montero Calle, Ana
A1 Solís Fernández, Guillermo
A1 Sánchez Martínez, Maricruz
A1 Rodríguez Salas, Nuria
A1 Cerón, María Ángeles
A1 Fernández Díez, Servando
A1 Domínguez Muñóz, Gemma
A1 Ríos, Vivian de los
A1 Peláez García, Alberto, Alberto
A1 Guzmán Aránguez, Ana Isabel
A1 Barderas Manchado, Rodrigo
AB Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer related death worldwide. Its diagnosis at early stages would significantly improve the survival of CRC patients. The humoral immune response has been demonstrated useful for cancer diagnosis, predating clinical symptoms up to 3 years. Here, we employed an in-depth seroproteomic approach to identify proteins that elicit a humoral immune response in CRC patients. The seroproteomic approach relied on the immunoprecipitation with patient-derived autoantibodies of proteins from CRC cell lines with different metastatic properties followed by LC-MS/MS. After bioinformatics, we focused on 31 targets of CRC autoantibodies. After WB and IHC validation, ERP44 and TALDO1 showed potential to discriminate disease-free and metastatic CRC patients, and time to recurrence of CRC patients in stage II. Using plasma samples of 30 healthy individuals, 28 premalignant individuals, and 32 CRC patients, nine out of 13 selected targets for seroreactive analysis showed significant diagnostic ability to discriminate either CRC patients or premalignant subjects from controls. Our results suggest that the here defined panel of CRC autoantibodies and their target proteins should be included in CRC blood-based biomarker panels to get a clinically useful blood-based diagnostic signature for CRC detection.Significance: Colorectal cancer is one of the deadliest cancer types mainly due to its late diagnosis. Its early diagnosis, therefore, is of great importance since it would significantly improve the survival of CRC patients. In our work, the in-depth seroproteomic analysis of colorectal cancer using isolated IgGs from colorectal cancer patients and controls and protein extract of colorectal cancer cells provide the identification of valuable biomarkers with diagnostic and prognostic ability of the disease.
PB Elsevier
SN 1874-3919
YR 2020
FD 2020-03
LK https://hdl.handle.net/20.500.14352/6047
UL https://hdl.handle.net/20.500.14352/6047
LA eng
NO Received 19 September 2019, Revised 2 January 2020, Accepted 5 January 2020, Available online 7 January 2020.
NO Ministerio de Economía y Competitividad de España (MINECO) - Programa Ramón y Cajal
NO AES-ISCIII program
NO Consejería de Educación, Juventud y Deporte de la Comunidad de Madrid
NO Fondo Social Europeo
NO Ministerio de Educación, Cultura y Deporte de España
NO The Flanders Research Foundation (FWO)
DS Docta Complutense
RD 28 abr 2024