RT Journal Article
T1 Anisakis simplex: Immunomodulatory effects of larval antigens on the activation of Toll like Receptors
A1 Zamora, Vega
A1 Andreu-Ballester, Juan Carlos
A1 Rodero Martínez, Marta
A1 Cuéllar Del Hoyo, María Del Carmen
AB Aims: The objective of this investigation is to evaluate the mechanisms Anisakis simplex employs to modify its host immune system, regarding the larval antigens interactions with Toll-Like-Receptors (TLRs).Methods and Results: In a previous study, we described that the stimulation of bone marrow derived dendritic cells (BMDCs) with A. simplex larval antigens drive an acute inflammatory response in BALB/c mice, but a more discrete and longer response in C57BL/6J. Moreover, when A. simplex larval antigens were combined with TLR agonists (TLR 1/2–9), they modified mainly TLR2, TLR4 and TLR9 agonists responses in both mice strains, and also TLR3, TLR5 and TLR7 in BALB/c. Antigen-presenting ability was analyzed by the detection of CD11c + cells expressing surface markers (CD80-86, MHC I-II), intracellular cytokines (IL-10, IL-12, TNF-α) and intracellular proteins (Myd88, NF-κβ) by Flow Cytometry. Secreted IL-10 was measured by ELISA.Conclusion: Our findings confirm not only that the host genetic basis plays a role in the development of a Th2/Th1/Treg response, but also it states A. simplex larval antigens present specific mechanisms to modify the innate response of the host. As allergies share common pathways with the immune response against this particular helminth, our results provide a better understanding into the specific mechanisms of A. simplex allergy related diseases.
PB Elsevier
SN 1567-5769
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/101663
UL https://hdl.handle.net/20.500.14352/101663
LA eng
NO Zamora V, Carlos Andreu-Ballester J, Rodero M, Cuéllar C. Anisakis simplex: Immunomodulatory effects of larval antigens on the activation of Toll like Receptors. Int Immunopharmacol. 2021 Nov;100:108120. doi: 10.1016/j.intimp.2021.108120. Epub 2021 Sep 17. PMID: 34537480.
NO Fundación Ramón Areces
DS Docta Complutense
RD 24 abr 2025