RT Journal Article
T1 Enzymatic/Immunoassay Dual-Biomarker Sensing Chip: Towards Decentralized Insulin/Glucose Detection
A1 Vargas, Eva
A1 Teymourian, Hazhir
A1 Tehrani, Farshad
A1 Eksin, Ece
A1 Sánchez Tirado, Esther
A1 Warren, Paul
A1 Erdem, Arzum
A1 Dassau, Eyal
A1 Wang, Joseph
AB Performing bioassay formats based on enzyme and antibody recognition reactions with a single detection chip remains an unmet challenge owing to the different requirements of such bioassays. Herein, we describe a dual-marker biosensor chip, integrating enzyme and antibody-based assays for simultaneous electrochemical measurements of insulin (I) and glucose (G). Simultaneous G/I sensing has been realized by addressing key fabrication and operational challenges associated with the different assay requirements and surface chemistry. The I immunosensor relies on a peroxidase-labeled sandwich immunoassay, while G is monitored through reaction with glucose oxidase. The dual diabetes biomarker chip offers selective and reproducible detection of picomolar I and millimolar G concentrations in a single microliter sample droplet within less than 30 min, including direct measurements in whole blood and saliva samples. The resulting integrated enzymatic-immunoassay biosensor chip opens a new realm in point-of-care multiplexed biomarker detection.
PB Wiley
SN 1433-7851
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/95345
UL https://hdl.handle.net/20.500.14352/95345
LA eng
NO Vargas E, Teymourian H, Tehrani F, Eksin E, Sánchez-Tirado E, Warren P, Erdem A, Dassau E, Wang J. Enzymatic/Immunoassay Dual-Biomarker Sensing Chip: Towards Decentralized Insulin/Glucose Detection. Angew Chem Int Ed. 2019 May 6; 58(19):6376-79
NO Leona M. and Harry B. Helmsley Charitable Trust
NO The Scientific and Technological Research Council of Turkey (Turkey)
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 5 abr 2025