RT Journal Article
T1 Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells
T2 Combinación de cultivo celular de baja densidad, seguimiento unicelular y patch-clamp para monitorizar el comportamiento de células madre neurales cerebelosas murinas postnatales
A1 Menéndez-Méndez, Aida
A1 Paniagua-Herranz, Lucía
A1 Olivos Ore, Luis Alcides
A1 Gómez Villafuertes, María Rosa
A1 Rodríguez Artalejo, Antonio
A1 Pérez Sen, Raquel
A1 García Delicado, Esmerilda
A1 Ortega De La O, Felipe
AB Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs maintain their neurogenic nature giving rise, in the same relative proportions that exist in vivo, to the neuronal progeny generated by the three postnatal cerebellar neurogenic niches. This protocol describes the identification of the nature of the progeny through both post-imaging immunocytochemistry and patch-clamp recordings. For complete details on the use and execution of this protocol, please refer to Paniagua-Herranz et al. (2020b).
PB Cell Press
SN 2666-1667
YR 2021
FD 2021-12-17
LK https://hdl.handle.net/20.500.14352/94829
UL https://hdl.handle.net/20.500.14352/94829
LA eng
NO Government of Madrid (PR65/19-22453)
NO Spanish Ministerio de Ciencia, Innovacioín y Universidades MCIU, (PID2019-109155RB-I00, BFU2015-70067REDC).
DS Docta Complutense
RD 22 ene 2026