RT Journal Article
T1 Antifungal Synergy: Mechanistic Insights into the R-1-R Peptide and Bidens pilosa Extract as Potent Therapeutics against Candida spp. through Proteomics
A1 Parra Giraldo, Claudia Marcela
A1 Bravo-Chaucanés, Patricia
A1 Monteoliva Díaz, Lucía
A1 Gil, Concha
A1 de la Cámara Fuentes, Samuel
A1 Vargas-Casanova, Yerly
A1 Rivera-Monroy, Zuly Jenny
A1 Modesti Costa, Geison
A1 García Castañeda, Javier Eduardo
AB Previous reports have demonstrated that the peptide derived from LfcinB, R-1-R, exhibits anti-Candida activity, which is enhanced when combined with an extract from the Bidens pilosa plant. However, the mechanism of action remains unexplored. In this research, a proteomic study was carried out, followed by a bioinformatic analysis and biological assays in both the SC5314 strain and a fluconazole-resistant isolate of Candida albicans after incubation with R-1-R. The proteomic data revealed that treatment with R-1-R led to the up-regulation of most differentially expressed proteins compared to the controls in both strains. These proteins are primarily involved in membrane and cell wall biosynthesis, membrane transport, oxidative stress response, the mitochondrial respiratory chain, and DNA damage response. Additionally, proteomic analysis of the C. albicans parental strain SC5314 treated with R-1-R combined with an ethanolic extract of B. pilosa was performed. The differentially expressed proteins following this combined treatment were involved in similar functional processes as those treated with the R-1-R peptide alone but were mostly down-regulated (data are available through ProteomeXchange with identifier PXD053558). Biological assays validated the proteomic results, evidencing cell surface damage, reactive oxygen species generation, and decreased mitochondrial membrane potential. These findings provide insights into the complex antifungal mechanisms of the R-1-R peptide and its combination with the B. pilosa extract, potentially informing future studies on natural product derivatives.
PB MDPI
YR 2024
FD 2024-08-16
LK https://hdl.handle.net/20.500.14352/131286
UL https://hdl.handle.net/20.500.14352/131286
LA eng
NO Vargas-Casanova, Y.; Bravo-Chaucanés, C.P.; Fuentes, S.d.l.C.; Martinez-Lopez, R.; Monteoliva, L.; Gil, C.; Rivera-Monroy, Z.J.; Costa, G.M.; Castañeda, J.E.G.; Parra-Giraldo, C.M. Antifungal Synergy: Mechanistic Insights into the R-1-R Peptide and Bidens pilosa Extract as Potent Therapeutics against Candida spp. through Proteomics. Int. J. Mol. Sci. 2024, 25, 8938. https:// doi.org/10.3390/ijms25168938
DS Docta Complutense
RD 19 mar 2026