RT Journal Article
T1 Comparison of cell mechanical measurements provided by Atomic ForceMicroscopy (AFM) and Micropipette Aspiration (MPA)
A1 Daza, Rafael
A1 González Bermúdez, Blanca
A1 Cruces, Julia
A1 Fuente del Rey, Mónica de la
A1 Plaza, Gustavo R.
A1 Arroyo Hernández, María
A1 Elices Calafat, Manuel
A1 Pérez Rigueiro, José
A1 Guinea, Gustavo V.
AB A comparative analysis of T-lymphocyte mechanical data obtained from Micropipette Aspiration (MPA) andAtomic Force Microscopy (AFM) is presented. Results obtained byfitting the experimental data to simple Hertzand Theret models led to non-Gaussian distributions and significantly different values of the elastic moduliobtained by both techniques. The use of more refined models, taking into account thefinite size of cells (sim-plified double contact and Zhou models) reduces the differences in the values calculated for the elastic moduli.Several possible sources for the discrepancy between the techniques are considered. The analysis suggests thatthe local nature of AFM measurements compared with the more general character of MPA measurementsprobably contributed to the differences observed.
PB Elsevier
SN 1878-0180
YR 2019
FD 2019-04-08
LK https://hdl.handle.net/20.500.14352/13698
UL https://hdl.handle.net/20.500.14352/13698
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid
DS Docta Complutense
RD 5 abr 2025