RT Journal Article
T1 Detection of caprine paratuberculosis (Johne's disease) in pre- and post-vaccinated herds: morphological diagnosis, lesion grading, and bacterial identification
A1 Plamenova Stefanova, Elena
A1 Sierra, Eva
A1 Fernández, Antonio
A1 Quesada Canales, Oscar
A1 Paz Sánchez, Yania
A1 Colom Rivero, Ana
A1 Espinosa de los Monteros, Antonio
A1 Herráez, Pedro
A1 Domínguez Rodríguez, Lucas José
A1 Bezos Garrido, Javier
A1 Pérez Sancho, Marta
A1 Moreno, Inmaculada
A1 Risalde, María A.
A1 Andrada, Marisa
AB Samples from the mesenteric lymph nodes (MS LNs) and ileocecal valves (ICV) of 105 goats, comprising 61 non-vaccinated and 44 vaccinated against Mycobacterium avium subspecies paratuberculosis (MAP), were collected at slaughter from a farm with a confirmed history of paratuberculosis (PTB). These goats had subclinical infections. PTB-compatible lesions in the MS LNs, ICV lamina propria (LP), and Peyer's patches (PPs) were graded separately. Furthermore, the load of acid-fast bacilli was quantified using Ziehl-Neelsen staining (ZN), MAP antigens by immunohistochemistry (IHC), and MAP DNA by PCR targeting the IS900 sequence. Gross PTB-compatible lesions were found in 39% of the goats, with 31.72% vaccinated (V) and 68.29% non-vaccinated (nV). Histopathological lesions induced MAP were observed in 58% of the animals, with 36.07% vaccinated and 63.93% non-vaccinated. The inclusion of histopathology as a diagnostic tool led to a 28% increase in diagnosed cases in MS LNs and 86.05% in ICV. Grade IV granulomas with central mineralization and necrosis were the most common lesions in MS LNs. In the ICV, mild granulomatous enteritis with multifocal foci of epithelioid macrophages was predominant, occurring more frequently in the PPs than in the LP. Furthermore, statistical differences in the presence of histopathological lesions between vaccinated and non-vaccinated goats were noted in MS LNs, ICV LPs, and ICV PPs. Non-vaccinated animals showed higher positivity rates in ZN, IHC, and PCR tests, underscoring the benefits of anti-MAP vaccination in reducing PTB lesions and bacterial load in target organs. Our findings emphasize the necessity of integrating gross and histopathological assessments with various laboratory techniques for accurate morphological and etiological diagnosis of PTB in both vaccinated and non-vaccinated goats with subclinical disease. However, further studies are required to refine sampling protocols for subclinical PTB in goats to enhance the consistency of diagnostic tools.
PB Frontiers
YR 2024
FD 2024-07-31
LK https://hdl.handle.net/20.500.14352/107852
UL https://hdl.handle.net/20.500.14352/107852
LA eng
NO Stefanova EP, Sierra E, Fernandez A, ´ Quesada-Canales O, Paz-Sanchez Y, ´ Colom-Rivero A, Espinosa de los Monteros A, Herraez P, Domínguez L, Bezos J, ´ Perez-Sancho M, Moreno I, Risalde MA and ´ Andrada M (2024) Detection of caprine paratuberculosis (Johne’s disease) in pre- and post-vaccinated herds: morphological diagnosis, lesion grading, and bacterial identification. Front. Vet. Sci. 11:1395928. doi: 10.3389/fvets.2024.1395928
NO Fundación CajaCanarias
NO Fundación "la Caixa"
NO Consejería de Economía, Conocimiento y Empleo (Canarias)
NO Canarian Agency for Research, Innovation and Information Society
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 9 abr 2025