RT Journal Article
T1 Cholesterol stimulates and ceramide inhibits Sticholysin II-induced pore formation in complex bilayer membranes
A1 Alm, Ida
A1 García Linares, Sara
A1 Gavilanes, José G.
A1 Martínez del Pozo, Álvaro
A1 Slotte, J. Peter
AB The pore forming capacity of Sticholysin II (StnII; isolated from Stichodactyla helianthus) in bilayer membranes containing 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), palmitoylsphingomyelin (PSM) and either cholesterol or palmitoyl ceramide (PCer) has been examined. The aim of the study was to elucidate how the presence of differently ordered PSM domains affected StnII oligomerization and pore formation. Cholesterol is known to enhance pore formation by StnII, and our results confirmed this and provide kinetic information for the process. The effect of cholesterol on bilayer permeabilization kinetics was concentration-dependent. In the concentration regime used (2.5–10 nmol cholesterol in POPC:PSM 80:20 by nmol), cholesterol also increased the acyl chain order in the fluid PSM domain and thus decreased bilayer fluidity, suggesting that fluidity per se was not responsible for cholesterol's effect. Addition of PCer (2.5–10 nmol) to the POPC:PSM (80:20 by nmol) bilayers attenuated StnII-induced pore formation, again in a concentration-dependent fashion. This addition also led to the formation of a PCer-rich gel phase. Addition of cholesterol to PCer-containing membranes could partially reduce the inhibitory effect of PCer on StnII pore formation. We conclude that the physical state of PSM (as influenced by either cholesterol or PCer) affected StnII binding and pore formation under the conditions examined.
PB Elsevier
SN 0006-3002
YR 2015
FD 2015-04
LK https://hdl.handle.net/20.500.14352/23386
UL https://hdl.handle.net/20.500.14352/23386
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Sigrid Juselius Foundation
NO Åbo Akademi Foundation
DS Docta Complutense
RD 29 abr 2024