RT Journal Article
T1 Screening and Identification of Potential Sex-AssociatedSequences in Danio rerio
A1 Luzio, Ana
A1 Coimbra, Ana M.
A1 Benito Jiménez, César
A1 Fontaínhas Fernandes, Antonio A.
A1 Matos, Manuela
AB Current knowledge on zebrafish (Danio rerio) suggests that sex determination has a polygenic genetic basis in this species, although environmental factors may also be involved. This study aimed to identify sex-associated genomic regions using two different marker systems: inter-simple sequence repeats (ISSRs) and random-amplified polymorphic DNA (RAPDs). Two bulks were constructed: one with DNA from zebrafish females and the other from males; then, a total of 100 ISSR and 280 RAPD primers were tested. Three DNA fragments presenting sexual dimorphism (female-linked: OPA17436 and OPQ191027 ; male-linked: OPQ19951 ) were determined from sequential analysis of the bulks followed by assessment in individuals. These fragments were cloned and convert into the following sequenced characterized amplified regions (SCAR): DrSM_F1, DrSM_F2, and DrSM_M, which share identities with sequences located in chromosomes 2, 3, and 11 (Zv9), respectively. Using these potential markers in zebrafish samples it was possible to correctly identify 80% of the males (DrSM_M) and 100% of the females (DrSM_F1 + DrSM_F2) in the analyzed population.
PB Wiley Online Library
SN 1040-452X
YR 2015
FD 2015-06-16
LK https://hdl.handle.net/20.500.14352/23361
UL https://hdl.handle.net/20.500.14352/23361
LA eng
NO Portuguese Foundation for Science and Technology
DS Docta Complutense
RD 9 may 2025