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oai:docta.ucm.es:20.500.14352/1000132024-02-14T01:20:14Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Piñeiro, David author González, Víctor M author Salinas, Matilde author Martín, M. Elena author Hernández Jiménez, Macarena author 2007-10 Changes to the translational machinery that occur during apoptosis have been described in the last few years. The two principal ways in which translational factors are modified during apoptosis are: (i) changes in protein phosphorylation and (ii) specific proteolytic cleavages. Taxol, a member of a new class of anti-tubulin drugs, is currently used in chemotherapeutic treatments of different types of cancers. We have previously demonstrated that taxol induces calpain-mediated apoptosis in NIH3T3 cells [Piñeiro et al., Exp. Cell Res., 2007, 313:369–379]. In this study we found that translation was significantly inhibited during taxol-induced apoptosis in these cells. We have studied the phosphorylation status and expression levels of eIF2a, eIF4E, eIF4G and the regulatory protein 4E-BP1, all of which are implicated in translation regulation. We found that taxol treatment did not induce changes in eIF2α phosphorylation, but strongly decreased eIF4G, eIF4E and 4E-BP1 expression levels. MDL28170, a specific inhibitor of calpain, prevented reduction of eIF4G, but not of eIF4E or 4E-BP1 levels. Moreover, the calpain inhibitor did not block taxol-induced translation inhibition. All together hese findings demonstrated that none of these factors are responsible for the taxol-induced protein synthesis inhibition. On the contrary, taxol treatment increased elongation factor eEF2 phosphorylation in a calpain-independent manner, supporting a role for eEF2 in taxol-induced translation inhibition. Piñeiro D, González VM, Hernández-Jiménez M, Salinas M, Martín ME. Translation regulation after taxol treatment in NIH3T3 cells involves the elongation factor (eEF)2. Exp Cell Res. 2007 Oct 15;313(17):3694-706. doi: 10.1016/j.yexcr.2007.07.025. Epub 2007 Aug 1. PMID: 17825817. 0014-4827 10.1016/j.yexcr.2007.07.025 https://hdl.handle.net/20.500.14352/100013 https://www.sciencedirect.com/science/article/pii/S0014482707003618?via%3Dihub Translation regulation after taxol treatment in NIH3T3 cells involves the elongation factor (eEF)2