2026-06-28T10:08:42Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1003982025-03-18T14:30:54Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Cuesta, Fernando de la author Barderas, Maria author Calvo, Enrique author Zubiri, Irene author Maroto, Aroa author Darde, Veronica author Martin-Rojas, Tatiana author Gil Dones, Félix author Posada-Ayala, Maria author Tejerina, Teresa author Lopez, Juan author Vivanco Martínez, Fernando author Álvarez Llamas, Gloria 2024-02-08T12:19:44Z 2024-02-08T12:19:44Z 2012 De La Cuesta, Fernando, et al. «Secretome Analysis of Atherosclerotic and Non-Atherosclerotic Arteries Reveals Dynamic Extracellular Remodeling during Pathogenesis». Journal of Proteomics, vol. 75, n.o 10, junio de 2012, pp. 2960-71. https://doi.org/10.1016/j.jprot.2011.12.005. 1874-3919 10.1016/j.jprot.2011.12.005 https://hdl.handle.net/20.500.14352/100398 https://doi.org/10.1016/j.jprot.2011.12.005 Aims: Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. This study was aimed to the analysis of human arterial tissue secretome and to the quantitative comparison of healthy and atherosclerotic secretome to discover proteins with key roles in atherosclerosis development. Methods and results: Secretomes from three biological replicates of human atherosclerotic coronary arteries (APC), preatherosclerotic coronaries (PC) and mammaries (M) were analyzed by LC-MS/MS. The identified proteins were submitted to Ingenuity Pathway Analysis (IPA) tool. Label-free MS/MS based quantification was performed and validated by immunohistochemistry. 64 proteins were identified in the 3 replicates of at least one of the 3 groups and 15 secreted proteins have not been previously reported in plasma. Four proteins were significantly released in higher amounts by mammary tissue: gelsolin, vinculin, lamin A/C and phosphoglucomutase 5. Conclusion: The study of tissue secretome reveals key proteins involved in atherosclerosis which have not been previously reported in plasma. Novel proteins are here highlighted which could be potential therapeutic targets in clinical practice. eng Attribution-NonCommercial-NoDerivatives 4.0 International Secretome analysis of atherosclerotic and non-atherosclerotic arteries reveals dynamic extracellular remodeling during pathogenesis journal article 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