2026-06-29T02:37:51Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1004192024-09-13T16:48:06Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Fernandes Ribeiro, Ana Sofía author Leite Fernandes, Vitor Samuel author Martínez Sainz, María Del Pilar author Martínez-Sáenz, Ana author Pazos Rodríguez, María Ruth author Orensanz Muñoz, Luis Miguel author Recio Visedo, María Paz author Bustamante Alarma, Salvador author Carballido Rodríguez, Joaquín author García Sacristán, Albino author Prieto Ocejo, Dolores author Hernández Rodríguez, Medardo Vicente author 2013-07-12 Aims: The current study investigates the role played by bradykinin (BK) receptors in the contractility to the pig bladder neck smooth muscle. Methods: Bladder neck strips were mounted in myographs for isometric force recordings and BK receptors expression was also determined by immunohistochemistry. Results: B2 receptor expression was observed in the muscular layer and urothelium whereas B1 expression was consistent detected in urothelium. A strong B2 immunoreactivity was also observed within nerve fibers among smooth muscle bundles. On urothelium-denuded preparations basal tone, BK induced concentration-dependent contractions which were reduced in urothelium-intact samples, by extracellular Ca(2+) removal and by blockade of B2 receptors and voltage-gated Ca(2+) (VOC) and non-VOC channels, and increased by cyclooxygenase (COX) inhibition. On phenylephrine-precontracted denuded strips, under non-adrenergic non-cholinergic (NANC) conditions, electrical field stimulation-elicited frequency-dependent relaxations which were reduced by B2 receptor blockade. In urothelium-intact samples, the B1 receptor agonist kallidin promoted concentration-dependent relaxations which were reduced by blockade of B1 receptors, COX, COX-1 and large-conductance Ca(2+) -activated K(+) (BKCa ) channels and abolished in urothelium-denuded samples and in K(+) -enriched physiological saline solution-precontracted strips. Conclusions: These results suggest that BK produces contraction of pig bladder neck via smooth muscle B2 receptors coupled to extracellular Ca(2+) entry via VOC and non-VOC channels with a minor role for intracellular Ca(2+) mobilization. Facilitatory neuronal B2 receptors modulating NANC inhibitory neurotransmission and urothelial B1 receptors producing relaxation via the COX-1 pathway and BKCa channel opening are also demonstrated. Ribeiro ASF, Fernandes VS, Martínez MP, Martínez-Sáenz A, Pazos MR, Orensanz LM, et al. Neuronal and non-neuronal bradykinin receptors are involved in the contraction and/or relaxation to the pig bladder neck smooth muscle: Bradykinin and Pig Bladder Neck Tension. Neurourol Urodynam 2014;33:558–65. https://doi.org/10.1002/nau.22434. 0733-2467 10.1002/nau.22434 https://hdl.handle.net/20.500.14352/100419 https://doi.org/10.1002/nau.22434 Neuronal and non-neuronal bradykinin receptors are involved in the contraction and/or relaxation to the pig bladder neck smooth muscle