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oai:docta.ucm.es:20.500.14352/1004192024-09-13T16:48:06Zcom_20.500.14352_14col_20.500.14352_15

Neuronal and non-neuronal bradykinin receptors are involved in the contraction and/or relaxation to the pig bladder neck smooth muscle Fernandes Ribeiro, Ana Sofía Leite Fernandes, Vitor Samuel Martínez Sainz, María Del Pilar Martínez-Sáenz, Ana Pazos Rodríguez, María Ruth Orensanz Muñoz, Luis Miguel Recio Visedo, María Paz Bustamante Alarma, Salvador Carballido Rodríguez, Joaquín García Sacristán, Albino Prieto Ocejo, Dolores Hernández Rodríguez, Medardo Vicente Aims: The current study investigates the role played by bradykinin (BK) receptors in the contractility to the pig bladder neck smooth muscle. Methods: Bladder neck strips were mounted in myographs for isometric force recordings and BK receptors expression was also determined by immunohistochemistry. Results: B2 receptor expression was observed in the muscular layer and urothelium whereas B1 expression was consistent detected in urothelium. A strong B2 immunoreactivity was also observed within nerve fibers among smooth muscle bundles. On urothelium-denuded preparations basal tone, BK induced concentration-dependent contractions which were reduced in urothelium-intact samples, by extracellular Ca(2+) removal and by blockade of B2 receptors and voltage-gated Ca(2+) (VOC) and non-VOC channels, and increased by cyclooxygenase (COX) inhibition. On phenylephrine-precontracted denuded strips, under non-adrenergic non-cholinergic (NANC) conditions, electrical field stimulation-elicited frequency-dependent relaxations which were reduced by B2 receptor blockade. In urothelium-intact samples, the B1 receptor agonist kallidin promoted concentration-dependent relaxations which were reduced by blockade of B1 receptors, COX, COX-1 and large-conductance Ca(2+) -activated K(+) (BKCa ) channels and abolished in urothelium-denuded samples and in K(+) -enriched physiological saline solution-precontracted strips. Conclusions: These results suggest that BK produces contraction of pig bladder neck via smooth muscle B2 receptors coupled to extracellular Ca(2+) entry via VOC and non-VOC channels with a minor role for intracellular Ca(2+) mobilization. Facilitatory neuronal B2 receptors modulating NANC inhibitory neurotransmission and urothelial B1 receptors producing relaxation via the COX-1 pathway and BKCa channel opening are also demonstrated. 2024-02-08T12:36:25Z 2024-02-08T12:36:25Z 2024-02-08T12:36:25Z 2013-07-12 journal article https://hdl.handle.net/20.500.14352/100419 0733-2467 10.1002/nau.22434 eng info:eu-repo/grantAgreement/MICINN//PS09%2F00044/ES/MECANISMOS INVOLUCRADOS EN LA RELAJACION INDEPENDIENTE DE OXIDO NITRICO EN EL CUELLO DE LA VEJIGA URINARIA: IMPLICACION TERAPEUTICA EN LA INCONTINENCIA URINARIA PRODUCIDA POR DEFICIENCIA ESFINTERICA INTRINSECA/ info:eu-repo/grantAgreement/SFRH/BD/68460/2010 Ribeiro ASF, Fernandes VS, Martínez MP, Martínez-Sáenz A, Pazos MR, Orensanz LM, et al. Neuronal and non-neuronal bradykinin receptors are involved in the contraction and/or relaxation to the pig bladder neck smooth muscle: Bradykinin and Pig Bladder Neck Tension. Neurourol Urodynam 2014;33:558–65. https://doi.org/10.1002/nau.22434. restricted access Wiley Periodicals, Inc.