2026-06-27T12:21:46Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1007462025-08-06T14:25:46Zcom_20.500.14352_14col_20.500.14352_15

Peiró Vallejo, Concepción Matesanz, Nuria Nevado, Julián Lafuente, Nuria Cercas, Elena Azcutia Criado, Verónica Vallejo, Susana Rodríguez-Mañas, Leocadio Sánchez-Ferrer, Carlos F. 2024-02-09T09:18:24Z 2024-02-09T09:18:24Z 2003 Peiró, Concepción, et al. «Glycosylated Human Oxyhaemoglobin Activates Nuclear Factor‐ κ B and Activator Protein‐1 in Cultured Human Aortic Smooth Muscle». British Journal of Pharmacology, vol. 140, n.o 4, octubre de 2003, pp. 681-90. https://doi.org/10.1038/sj.bjp.0705483. 0007-1188 10.1038/sj.bjp.0705483 https://hdl.handle.net/20.500.14352/100746 1476-5381 https://doi.org/10.1038/sj.bjp.0705483 https://pubmed.ncbi.nlm.nih.gov/14504138/ 1 Diabetic vessels undergo structural changes that are linked to a high incidence of cardiovascular diseases. Reactive oxygen species (ROS) mediate cell signalling in the vasculature, where they can promote cell growth and activate redox-regulated transcription factors, like activator protein-1 (AP-1) or nuclear factor-kB (NF-kB), which are involved in remodelling and inflammation processes. Amadori adducts, formed through nonenzymatic glycosylation, can contribute to ROS formation in diabetes. 2 In this study, we analysed whether Amadori-modified human oxyhaemoglobin, glycosylated at either normal (N-Hb) or elevated (E-Hb) levels, can induce cell growth and activate AP-1 and NF-kB in cultured human aortic smooth muscle cells (HASMC). 3 E-Hb (1 nM–1 mM), but not N-Hb, promoted a concentration-dependent increase in cell size from nanomolar concentrations, although it failed to stimulate HASMC proliferation. At 10 nM, E-Hb stimulated both AP-1 and NF-kB activity, as assessed by transient transfection, electromobility shift assays or immunofluorescence staining. The effects of E-Hb resembled those of the proinflammatory cytokine tumour necrosis factor-a (TNF-a). E-Hb enhanced intracellular superoxide anions content and its effects on HASMC were abolished by different ROS scavengers. 4 In conclusion, E-Hb stimulates growth and activates AP-1 and NF-kB in human vascular smooth muscle by redox-sensitive pathways, thus suggesting a possible direct role for Amadori adducts in diabetic vasculopathy. eng restricted access Glycosylated human oxyhaemoglobin activates nuclear factor-jB and activator protein-1 in cultured human aortic smooth muscle journal article