2026-06-08T02:45:27Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1011292025-03-18T12:11:01Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Laurenti, Marco author Paez-Pérez, Miguel author Algarra González, Manuel author Alonso Cristobal, Paulino author López Cabarcos, Enrique author Méndez González, Diego author Rubio Retama, Benito Jorge author 2016-05-25 We developed a sensor for the detection of specific microRNA (miRNA) sequences that was based on graphene quantum dots (GQDs) and ssDNA-UCNP@SiO2. The proposed sensor exploits the interaction between the sp2 carbon atoms of the GQD, mainly π–π stacking, and the DNA nucleobases anchored on the upconversion nanoparticles (UCNPs). This interaction brings the GQD to the surface of the ssDNA-UCNP@SiO2 system, enhancing the upconversion emission. On the other hand, hybridization of the single-stranded DNA (ssDNA) chains anchored on the nanoparticles with their complementary miRNA sequences blocks the capacity of the UCNPs to interact with the GQD through π–π stacking. That gives as result a reduction of the fluorescent enhancement, which is dependent on the concentration of miRNA sequences. This effect was used to create a sensor for miRNA sequences with a detection limit of 10 fM. Laurenti M, Paez-Perez M, Algarra M, Alonso-Cristobal P, Lopez-Cabarcos E, Mendez-Gonzalez D, et al. Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection. ACS Appl Mater Interfaces 2016;8:12644–51. https://doi.org/10.1021/acsami.6b02361. 1944-8244 10.1021/acsami.6b02361 https://hdl.handle.net/20.500.14352/101129 https://doi.org/10.1021/acsami.6b02361 Enhancement of the Upconversion Emission by Visible-to-Near-Infrared Fluorescent Graphene Quantum Dots for miRNA Detection