2026-08-23T06:36:47Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1019092024-03-05T00:49:58Zcom_20.500.14352_14col_20.500.14352_15

Romero Martínez, Beatriz Juan Ferré, Lucía De Domínguez Rodríguez, Lucas José Álvarez Sánchez, Julio Bezos Garrido, Javier Velasco, Carlos Ortega, Javier Domínguez, Mercedes Ricón, Jaime Moreno, Inmaculada 2024-03-04T13:56:38Z 2024-03-04T13:56:38Z 2024-02-08 Velasco-Reinaldos C, Ortega J, Rincón-Fernández de la Puente J, Romero B*, de Juan L, Dominguez L, Dominguez M, Moreno I, Alvarez J and Bezos J. Effect of a recent intradermal test on the specificity of P22 ELISA for the diagnosis of caprine tuberculosis. Frontiers in veterinary science, 11:1-7. 2024. (A). ISSN: 2297-1769. Impact factor 2022: 3.200. Category: Veterinary Sciences, Quartile: 1, Position: 12 of 143. DOI: 10.3389/fvets.2024.1358413 2297-1769 10.3389/fvets.2024.1358413 https://hdl.handle.net/20.500.14352/101909 https://www.frontiersin.org/articles/10.3389/fvets.2024.1358413/full Caprine tuberculosis (TB) is a zoonotic disease caused by members of the Mycobacterium tuberculosis complex. TB eradication programs in goats are based on the single and comparative intradermal tuberculin tests (SITT and CITT, respectively). Antibody-based diagnostic techniques have emerged as potential diagnostic tools for TB. P22 ELISA has been previously evaluated using samples collected after the intradermal tuberculin tests to maximize the sensitivity, a phenomenon known as booster effect. However, there is no information available on whether the use of this diagnostic strategy could lead to a decrease of its specificity (Sp). The aim of the present study was to elucidate the interference effect of a recent CITT on the Sp of the P22 ELISA in serum and milk samples collected at different times after the CITT from a TB-free herd (n = 113). The number of reactors to P22 ELISA was significantly higher (p < 0.01) on serum samples collected 15 days post-CITT compared to day 0, showing a decrease in Sp from 99.1% (95% CI; 95.2–99.8%) to 88.5% (95% CI; 81.3–93.2%). The number of reactors and the quantitative values of P22 ELISA were significantly higher (p < 0.01) in serum samples compared to milk. No significant (p > 0.05) changes in the Sp of the P22 ELISA were observed throughout the different time samplings using milk No significant (p > 0.05) changes were observed on days 30 and 60 post-CITT. In conclusion, the booster effect strategy may significantly decrease the Sp of P22 ELISA in TB-free herds when serum samples are used but not when milk is tested. eng http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 International Effect of a recent intradermal test on the specificity of P22 ELISA for the diagnosis of caprine tuberculosis journal article