2026-06-26T20:12:30Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1027022024-04-05T00:19:50Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Martínez Ruiz, Antonio author Villanueva, Laura author González de Orduña, Cecilia author López-Ferrer, Daniel author Higueras, María Ángeles author Tarín, Carlos author Rodríguez Crespo, José Ignacio author Vázquez, Jesús author Lamas, Santiago editor Ignarro, Louis J. 2024-04-04T10:21:50Z 2024-04-04T10:21:50Z 2005-06-03 0027-84241091-6490 10.1073/pnas.0407294102 https://hdl.handle.net/20.500.14352/102702 Nitric oxide is implicated in a variety of signaling pathways in different systems, notably in endothelial cells. Some of its effects can be exerted through covalent modifications of proteins and, among these modifications, increasing attention is being paid to S-nitrosylation as a signaling mechanism. In this work, we show by a variety of methods (ozone chemiluminescence, biotin switch, and mass spectrometry) that the molecular chaperone Hsp90 is a target of S-nitrosylation and identify a susceptible cysteine residue in the region of the C-terminal domain that interacts with endothelial nitric oxide synthase (eNOS). We also show that the modification occurs in endothelial cells when they are treated with S-nitrosoL-cysteine and when they are exposed to eNOS activators. Hsp90 ATPase activity and its positive effect on eNOS activity are both inhibited by S-nitrosylation. Together, these data suggest that S-nitrosylation may functionally regulate the general activities of Hsp90 and provide a feedback mechanism for limiting eNOS activation. eng Attribution-NonCommercial-NoDerivatives 4.0 International S-nitrosylation of Hsp90 promotes the inhibition of its ATPase and endothelial nitric oxide synthase regulatory activities journal article 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URL https://docta.ucm.es/bitstreams/339ff943-f817-4182-aec1-99236b304d1d/download File MD5 be1f6c0242d30cfe3d5035f61ec42aeb 426092 application/pdf 21 - 2005_Martinez-Ruiz_PNAS.pdf