2026-06-29T02:37:35Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1030472025-03-18T14:36:45Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Díaz Del Toro, Silvia author Aguilera, Ángeles author González de Figueras, Carolina author De Francisco Martínez, Patricia author Olsson, Sanna author Puente-Sánchez, Fernando author González-Pastor, José Eduardo 2024-04-12T09:58:46Z 2024-04-12T09:58:46Z 2022-06-23 10.3390/ijerph19137692 https://hdl.handle.net/20.500.14352/103047 2008-2304 https://www.mdpi.com/1660-4601/19/13/7692 Phytochelatins (PCs) are cysteine-rich small peptides, enzymatically synthesized from reduced glutathione (GSH) by cytosolic enzyme phytochelatin synthase (PCS). The open reading frame (ORF) of the phytochelatin synthase CaPCS2 gene from the microalgae Chlamydomonas acidophila was heterologously expressed in Escherichia coli strain DH5α, to analyze its role in protection against various abiotic agents that cause cellular stress. The transformed E. coli strain showed increased tolerance to exposure to different heavy metals (HMs) and arsenic (As), as well as to acidic pH and exposure to UVB, salt, or perchlorate. In addition to metal detoxification activity, new functions have also been reported for PCS and PCs. According to the results obtained in this work, the heterologous expression of CaPCS2 in E. coli provides protection against oxidative stress produced by metals and exposure to different ROS-inducing agents. However, the function of this PCS is not related to HM bioaccumulation. eng Attribution 4.0 International Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli journal article 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URL https://docta.ucm.es/bitstreams/47252cca-d45f-4796-a63e-2bad3a24c5d3/download File MD5 d2106a640ad6d9a8a63cec1c19195976 3259438 application/pdf Díaz, S. et al. 2022. Heterologous Expression of the Phytochelatin Synthase CaPCS2....pdf URL https://docta.ucm.es/bitstreams/911e96b7-16ef-4f29-a568-707ce7fea085/download File MD5 a1ad65dfd9eefcbcc70ada81b42a47c1 104497 text/plain Díaz, S. et al. 2022. Heterologous Expression of the Phytochelatin Synthase CaPCS2....pdf.txt