2026-06-27T10:23:18Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/1032012025-03-18T14:33:07Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Truchado Martín, Daniel Alejandro
author
Medrano Arranz, Carlos
author
Rincón, Sara
author
Zurita, Lucía
author
Ponz, Fernando
2024-04-17T17:57:36Z
2024-04-17T17:57:36Z
2024-04-07
0732-8893
10.1016/j.diagmicrobio.2024.116298
https://hdl.handle.net/20.500.14352/103201
https://doi.org/10.1016/j.diagmicrobio.2024.116298
Nanoparticles derived from plant viruses play an important role in nanomedicine due to their biocompatibility, self-assembly and easily-modifiable surface. In this study, we developed a novel platform for increasing antibody sensing using viral nanoparticles derived from turnip mosaic virus (TuMV) functionalized with SARS-CoV-2 receptor binding domain (RBD) through three different methods: chemical conjugation, gene fusion and the SpyTag/SpyCatcher technology. Even though gene fusion turned out to be unsuccessful, the other two constructs were proven to significantly increase antibody sensing when tested with saliva of patients with different infection and vaccination status to SARS-CoV-2. Our findings show the high potential of TuMV nanoparticles in the fields of diagnostics and immunodetection, being especially attractive for the development of novel antibody sensing devices.
eng
Attribution 4.0 International
Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD
journal article
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Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva.pdf
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Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva.pdf.txt