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oai:docta.ucm.es:20.500.14352/1033722025-03-18T12:51:27Zcom_20.500.14352_14col_20.500.14352_15

The cytotoxin α‐sarcin behaves as a cyclizing ribonuclease Lacadena García-Gallo, Francisco Javier Martínez Del Pozo, Álvaro Valle Lacadena Martínez Ruiz, Antonio Mancheño Gómez, José Miguel Oñaderra Sánchez, Mercedes Gavilanes Franco, José Gregorio The hydrolysis of adenylyl(3PC5P)adenosine (ApA) and guanylyl(3PC5P)adenosine (GpA) dinucleotides by the cytotoxic protein K-sarcin has been studied. Quantitative analysis of the reaction has been performed through reversephase chromatographic (HPLC) separation of the resulting products. The hydrolysis of the 3P-5P phosphodiester bond of these substrates yields the 2P-3P cyclic mononucleotide; this intermediate is converted into the corresponding 3P-monophosphate derivative as the final product of the reaction. The values of the apparent Michaelis constant (KM), kcat and kcat/ KM have also been calculated. The obtained results fit into a twostep mechanism for the enzymatic activity of K-sarcin and allow to consider this protein as a cyclizing RNase. z 1998 Federation of European Biochemical Societies. 2024-04-23T11:00:03Z 2024-04-23T11:00:03Z 2024-04-23T11:00:03Z 1998-03-06 journal article https://hdl.handle.net/20.500.14352/103372 0014-5793 1873-3468 10.1016/s0014-5793(98)00137-9 eng Lacadena Javier, Martı́nez del Pozo Alvaro, Lacadena Valle, Martı́nez-Ruiz Antonio, Mancheño José M, Oñaderra Mercedes and Gavilanes José G(1998), The cytotoxin α-sarcin behaves as a cyclizing ribonuclease, FEBS Letters, 424, doi: 10.1016/S0014-5793(98)00137-9 open access