2026-06-08T04:02:10Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1034472025-09-16T14:44:06Zcom_20.500.14352_14col_20.500.14352_15

Docta Complutense author Cabañas Morafraile, Esther author Saiz Ladera, Cristina author Nieto Jiménez, Cristina author Győrffy, Balázs author Nagy, Adam author Velasco Díez, Guillermo author Pérez Segura, Pedro author Ocaña, Alberto 2024-04-24T12:57:01Z 2024-04-24T12:57:01Z 2023 1718-7729 10.3390/curroncol30030196 https://hdl.handle.net/20.500.14352/103447 https://doi.org/10.3390/curroncol30030196 https://www.mdpi.com/1718-7729/30/3/196 Despite the impressive results obtained with immunotherapy in several cancer types, a significant fraction of patients remains unresponsive to these treatments. In colorectal cancer (CRC), B-RafV600 mutations have been identified in 8–15% of the patients. In this work we interrogated a public dataset to explore the surfaceome of these tumors and found that several genes, such as GP2, CLDN18, AQP5, TM4SF4, NTSR1, VNN1, and CD109, were upregulated. By performing gene set enrichment analysis, we also identified a striking upregulation of genes (CD74, LAG3, HLA-DQB1, HLA-DRB5, HLA-DMA, HLA-DMB, HLA-DPB1, HLA-DRA, HLA-DOA, FCGR2B, HLA-DQA1, HLA-DRB1, and HLA-DPA1) associated with antigen processing and presentation via MHC class II. Likewise, we found a strong correlation between PD1 and PD(L)1 expression and the presence of genes encoding for proteins involved in antigen presentation such as CD74, HLA-DPA1, and LAG3. Furthermore, a similar association was observed for the presence of dendritic cells and macrophages. Finally, a low but positive relationship was observed between tumor mutational burden and neoantigen load. Our findings support the idea that a therapeutic strategy based on the targeting of PD(L)1 together with other receptors also involved in immuno-modulation, such as LAG3, could help to improve current treatments against BRAF-mutated CRC tumors. eng Attribution 4.0 International Mapping Immune Correlates and Surfaceome Genes in BRAF Mutated Colorectal Cancers journal article 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URL https://docta.ucm.es/bitstreams/b5500468-b784-48dc-8ae6-4e2d487a1025/download File MD5 b061708fa33de729b3d2e4bff4df1944 14905819 application/pdf Mapping Immune Correlates and Surfaceome Genes.pdf URL https://docta.ucm.es/bitstreams/2121e933-2053-4e5d-aa2c-f6c29254e404/download File MD5 b5f7fdb5b40ef3f1ace38969099190a7 48297 text/plain Mapping Immune Correlates and Surfaceome Genes.pdf.txt