2026-06-08T10:43:20Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1035332024-12-21T01:00:36Zcom_20.500.14352_14col_20.500.14352_15

Peripheral and intestinal T lymphocyte subsets in dogs with chronic inflammatory enteropathy Agulla Pérez, Beatriz Villaescusa Fernández, Alejandra Sainz Rodríguez, Ángel Díaz-Regañón Fernández, David Rafael Rodríguez Franco, Fernando Calleja Bueno, Lydia Olmeda, Patricia García-Sancho Téllez, Mercedes Guadalupe 636.09 Flow cytometry IBD Intraepithelial lymphocytes Lamina propria lymphocytes Peripheral blood lymphocytes T lymphocytes Veterinaria 3109 Ciencias Veterinarias Abbreviations: BCS, body condition score; BSA, bovine serum albumin; CBC, complete blood count; CCECAI, clinical canine chronic enteropathy activity index; CD, clusters of differentiation;CIBDAI, clinical IBD activity index; CIE, chronic inflammatory enteropathy; CVMTH, Complutense Veterinary Medicine Teaching Hospital; DTT, 1,4-dithiothreitol; EDTA,ethylenediaminetetraacetic acid; FBS, fetal bovine serum; FC, flow cytometry; GALT, gut-associated lymphoid tissue; GI, gastrointestinal; HBSS, Hank's Buffered Salt Solution; HC, healthycontrol; IBD, inflammatory bowel disease; IEL, intraepithelial lymphocyte; IFN-γ, interferon-gamma; IL, interleukin; IRE, immunosuppressant-responsive enteropathy; LPL, lamina proprialymphocyte; mAbs, monoclonal antibodies; NLR, neutrophil-to-lymphocyte ratio; PBL, peripheral blood lymphocyte; PBMCs, peripheral blood mononuclear cells; PBS, phosphate-buffered saline;PLR, platelet-to-lymphocyte ratio; SII, systemic immune-inflammation index; SRE, steroid-responsive enteropathy; Tc, T cytotoxic; Th, T helper; TLI, trypsin-like immunoreactivity; Treg,regulatory T cells; WSAVA, World Small Animal Veterinary Association. Background: Dysregulated T lymphocyte response is thought to play a key role inchronic intestinal inflammation (CIE). Objectives: To evaluate the presence of changes in peripheral and intestinal T lym-phocyte subsets and to describe potential immune and inflammatory biomarkers indogs with CIE. Animals: Sixteen healthy dogs and 26 dogs were diagnosed with CIE. Methods: Prospective case-control study evaluating peripheral and intestinal T lym-phocytes using flow cytometry and inflammatory markers obtained from completeblood cell counts. Results: Dogs with CIE had higher peripheral activated T helper (Th) lymphocytes(87/μL [18-273] CIE, 44/μL [16-162] healthy control (HC,P=.013) and regulatory Tcells (Treg; 108/μL [2-257] CIE, 34/μL [1-114] HC,P=.004). In the intestinal epithe-lium, CIE dogs presented lower percentages of Th (4.55% [1.75-18.67] CIE, 8.77%[3.79-25.03] HC,P=.002), activated Th cells (0.16% [0.02-0.83] CIE, 0.33%[0.05-0.57] HC,P=.03) and CD4/CD8 ratio (0.08 [0.02-0.39] CIE, 0.21 [0.07-0.85]HC,P=.003). Conversely, higher percentage of activated T cytotoxic cells (20.24%[3.12-77.12] CIE, 12.32% [1.21-39.22] HC,P=.04) and interferon-gamma (IFN-γ)producing T lymphocytes (7.36% [0.63-55.83] CIE, 1.44% [0.00-10.56] HC,P=.01) within the epithelium was observed. In the lamina propria the percentage of Treglymphocytes was higher (6.02% [1.00-21.48] CIE, 3.52% [0.18-10.52] HC,P=.02). Conclusions and Clinical Importance: Systemic and intestinal immune alterationsoccur in dogs with CIE suggesting that blood IFN-γproducing T lymphocytes and the systemic immune-inflamation index (SII) could potentially serve as biomarkers for the disease. Universidad Complutense de Madrid Depto. de Medicina y Cirugía Animal Fac. de Veterinaria TRUE pub 2024-04-25T17:10:38Z 2024-04-25T17:10:38Z 2024-03-20 journal article VoR https://hdl.handle.net/20.500.14352/103533 0891-6640 10.1111/jvim.17036 1939-1676 eng info:eu-repo/grantAgreement/UCM/PR26/-16-20319 info:eu-repo/grantAgreement/UCM/PR75/18-21623 info:eu-repo/grantAgreement/UCM/PR87/19-22606 Agulla B, Villaescusa A, Sainz A, et al. ´ Peripheral and intestinal T lymphocyte subsets in dogs with chronic inflammatory enteropathy. J Vet Intern Med. 2024; 1‐12. doi:10.1111/jvim.17036 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ open access application/pdf application/pdf Wiley