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00925njm 22002777a 4500 dc Cañón Beltrán, Karina Esperanza author García García, Rosa María author Cajas Suárez, Yulia Nathaly author Fierro, Natacha author Lorenzo González, Pedro Luis author Arias Álvarez, María author 2023-10-24 In vitro maturation (IVM) system is an alternative method to superovulation protocols to obtain mature oocytes. Epidermal Growth Factor (EGF) and Insulin-like Growth Factor I (IGF-I) have been widely used in IVM medium in different species. Although the guinea pig is a valuable animal model for reproductive studies, IVM is rarely used. We aimed to establish a suitable in vitro production system using EGF and/or IGF-I during IVM to improve oocyte competence. Firstly, immunolocalization of EGF and IGF-I receptors in the ovary was assessed. An IVM dose-response experiment was performed with cumulus-oocyte complexes (COCs) supplemented with: 1) EGF [0, 10, 50, 100 ng/mL or 10% fetal calf serum (FCS)]; 2) IGF-I [0, 50, 100, 200 ng/mL or 10% FCS]; or 3) the concentrations of EGF and IGF-I which showed the best IVM index in the previous experiments, with or without Fetal Calf Serum (FCS). Cortical granule and mitochondria distribution patterns were determined in in vivo and in vitro-matured oocytes for the first time in this species. Apoptotic rate after IVM and oocyte competence by in vitro embryo development were evaluated. Immunohistochemistry results showed positive immunostaining of EGF and IGF receptors in corpus luteum, oocytes, granulosa and theca cells in follicles in all stages of development. Supplementation of IVM medium with 50 ng/mL EGF or 100 ng/mL IGF-I or their combination with FCS successfully led to oocyte nuclear and cytoplasmic maturation and reduced the apoptotic rate. Both growth factors improved oocyte competence during IVM in this species since early embryos were in vitro developed, showing better results when FCS was used in the IVM medium. Karina Cañón-Beltrán, Rosa M. García-García, Yulia N. Cajas, Natacha Fierro, Pedro L. Lorenzo, María Arias-Álvarez, Improvement of oocyte competence and in vitro oocyte maturation with EGF and IGF-I in Guinea pig model, Theriogenology, Volume 214, 2024, Pages 206-214, https://doi.org/10.1016/j.theriogenology.2023.10.029. 0093-691X 10.1016/j.theriogenology.2023.10.029 https://hdl.handle.net/20.500.14352/104330 1879-3231 https://doi.org/10.1016/j.theriogenology.2023.10.029 Improvement of oocyte competence and in vitro oocyte maturation with EGF and IGF-I in Guinea pig model