2026-06-29T02:38:05Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1044302025-03-18T14:46:47Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Valdivia Lara, Edgar Guillermo author Alonso Miguel, Daniel author Pérez Alenza, María De Los Dolores author Zimmermann, Anna Barbara Emilia author Schaafsma, Evelien author Kolling IV, Fred W. author Barreno San Antolín, Lucía author Alonso Díez, Ángela author Beiss, Veronique author Affonso de Oliveira, Jessica Fernanda author Suárez Redondo, María author Fiering, Steven author Steinmetz, Nicole F. author Vom Berg, Johannes author Peña Fernández, Laura Luisa author Arias Pulido, Hugo author 2023-09-08 The lack of optimal models to evaluate novel agents is delaying the development of effective immunotherapies against human breast cancer (BC). In this prospective open label study, we applied neoadjuvant intratumoral immunotherapy with empty cowpea mosaic virus-like particles (eCPMV) to 11 companion dogs diagnosed with canine mammary cancer (CMC), a spontaneous tumor resembling human BC. We found that two neoadjuvant intratumoral eCPMV injections resulted in tumor reduction in injected tumors in all patients and in noninjected tumors located in the ipsilateral and contralateral mammary chains of injected dogs. Tumor reduction was independent of clinical stage, tumor size, histopathologic grade, and tumor molecular subtype. RNA-seq-based analysis of injected tumors indicated a decrease in DNA replication activity and an increase in activated dendritic cell infiltration in the tumor microenvironment. Immunohistochemistry analysis demonstrated significant intratumoral increases in neutrophils, T and B lymphocytes, and plasma cells. eCPMV intratumoral immunotherapy demonstrated antitumor efficacy without any adverse effects. This novel immunotherapy has the potential for improving outcomes for human BC patients. Valdivia, G.; AlonsoMiguel, D.; Perez-Alenza, M.D.; Zimmermann, A.B.E.; Schaafsma, E.; Kolling, F.W., IV; Barreno, L.; Alonso-Diez, A.; Beiss, V.; Affonso de Oliveira, J.F.; et al. Neoadjuvant Intratumoral Immunotherapy with Cowpea Mosaic Virus Induces Local and Systemic Antitumor Efficacy in Canine Mammary Cancer Patients. Cells 2023, 12, 2241. https://doi.org/ 10.3390/cells12182241 10.3390/cells12182241 https://hdl.handle.net/20.500.14352/104430 2073-4409 https://doi.org/10.3390/cells12182241 https://pubmed.ncbi.nlm.nih.gov/37759464/ Neoadjuvant Intratumoral Immunotherapy with Cowpea Mosaic Virus Induces Local and Systemic Antitumor Efficacy in Canine Mammary Cancer Patients