2026-08-23T19:04:18Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1044562024-12-20T09:31:50Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author González Arto, Marta author Vicente Carrillo, Alejandro author Martínez Pastor, Felipe author Fernández Alegre, Estela author Roca, Jordi author Miró, Jordi author Rigau, Teresa author Rodríguez Gil, Joan Enric author Pérez Pé, Rosaura author Muiño Blanco, Teresa author Cebrián Pérez, José Álvaro author Casao, Adriana 2024-05-27T11:23:14Z 2024-05-27T11:23:14Z 2016-06-15 Marta González-Arto, Alejandro Vicente-Carrillo, Felipe Martínez-Pastor, Estela Fernández-Alegre, Jordi Roca, Jordi Miró, Teresa Rigau, Joan E. Rodríguez-Gil, Rosaura Pérez-Pé, Teresa Muiño-Blanco, José A. Cebrián-Pérez, Adriana Casao, Melatonin receptors MT1 and MT2 are expressed in spermatozoa from several seasonal and nonseasonal breeder species, Theriogenology, Volume 86, Issue 8, 2016, Pages 1958-1968, https://doi.org/10.1016/j.theriogenology.2016.06.016. 0093-691X 10.1016/j.theriogenology.2016.06.016 https://hdl.handle.net/20.500.14352/104456 1879-3231 http://dx.doi.org/10.1016/j.theriogenology.2016.06.016 Melatonin is a ubiquitous and multipurpose molecule, and one of its roles is to regulate reproduction in some seasonal mammals. Our group has previously reported the variation in the melatonin levels in ram seminal plasma along the year and identified MT1 and MT2 receptors in ram spermatozoa. The objective of this study was to elucidate whether the presence of melatonin receptors (MT1 and MT2) in the sperm plasma membrane, and melatonin in the seminal plasma is related to seasonal breeding. For this purpose, the presence of melatonin receptors and the levels of melatonin in seminal plasma have been examined in several species: donkey and stallion as long-day breeders; red deer as a wild, short-day, highly seasonal breeder (epididymal spermatozoa); bull as a conventional nonseasonal breeder; boar as a seasonal breeder under management techniques; and dog as possible a seasonal breeder not regulated by melatonin. We have detected measurable levels of melatonin in the seminal plasma of all ejaculated semen samples (from donkey, stallion, boar, bull, and dog). Also, and for the first time, we have demonstrated the presence of MT1 and MT2 melatonin receptors in the spermatozoa of all these species, regardless their type of reproduction or sperm source (ejaculated or epididymal), using indirect immunofluorescence techniques and Western blotting. Our findings suggest that melatonin and melatonin receptors may be universally distributed in the reproductive system of mammals and that the sperm melatonin receptors cells may not be necessarily related with seasonal reproduction. Furthermore, the presence of MT1 at the cytoplasmic droplet in immature ejaculated stallion spermatozoa found in one sample and epididymal red deer spermatozoa suggests that melatonin may be involved in specific functions during spermatogenesis and sperm maturation, like protecting spermatozoa from oxidative damage, this activity being mediated through these receptors. eng Attribution-NonCommercial-NoDerivatives 4.0 International Melatonin receptors MT1 and MT2 are expressed in spermatozoa from several seasonal and nonseasonal breeder species journal article 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URL https://docta.ucm.es/bitstreams/a0b7558a-1ee2-4dfd-bac7-afa55098971b/download File MD5 d98f88d0eefa9aa0502368b7ded36716 2121496 application/pdf González-Arto et al-2016-Theriogenology.pdf