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oai:docta.ucm.es:20.500.14352/1045592025-09-05T17:53:14Zcom_20.500.14352_14col_20.500.14352_15

Ferreras Colino, Elisa Moreno, Inmaculada Gortázar, Christian Sevilla, Iker Agulló Ros, Irene Domínguez Rodríguez, Lucas José Juste, Ramón Risalde, María A. Domínguez, Mercedes 2024-05-29T16:57:25Z 2024-05-29T16:57:25Z 2023 Elisa Ferreras-Colino, Inmaculada Moreno, Christian Gortázar, Iker Sevilla, Irene Agulló-Ros, Lucas Domínguez, Ramón Juste, Maria A. Risalde, Mercedes Domínguez, Oral immunization with heat-inactivated Mycobacterium bovis reduces local parasite dissemination and hepatic granuloma development in mice infected with Leishmania amazonensis, Research in Veterinary Science, Volume 162, 2023, 104963, https://doi.org/10.1016/j.rvsc.2023.104963. 0034-5288 10.1016/j.rvsc.2023.104963 https://hdl.handle.net/20.500.14352/104559 https://doi.org/10.1016/j.rvsc.2023.104963 Aiming to explore whether oral immunization with heat-inactivated Mycobacterium bovis (HIMB) protects mice against Leishmania infection, 18 female BALB/c mice were randomly assigned to the immunized group, that received oral HIMB, or the control group, and were infected by inoculation of 10,000 Leishmania amazonensis promastigotes in the footpad. Spleen culture was positive in 55.55% of immunized mice and in 100% of control mice (p = 0.082). The number of immunolabeled amastigotes number in the popliteal lymph node was lower in the immunized group (p = 0.009). The immunized group presented fewer mature granulomas in the liver (p = 0.005) and more Lys + macrophages (p = 0.002) and fewer CD3+ T lymphocytes (p < 0.001) per hepatic granuloma. We conclude that immunization with HIMB via the oral route limited local parasite dissemination and hepatic granuloma development in mice challenged with Leishmania amazonensis through stimulation of macrophages, which is compatible with trained immunity. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 International Oral immunization with heat-inactivated Mycobacterium bovis reduces local parasite dissemination and hepatic granuloma development in mice infected with Leishmania amazonensis journal article