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oai:docta.ucm.es:20.500.14352/1046072025-03-18T14:46:04Zcom_20.500.14352_14col_20.500.14352_15

Barcenas Peña, Alejandrina Dulare Devi, Pradeep Divakar Crespo De Las Casas, Ana María Núñez Zapata, Jano Lumbsch, H. Thorsten Grewe, Felix 2024-05-30T15:00:14Z 2024-05-30T15:00:14Z 2023-12-09 Barcenas-Peña A, Divakar PK, Crespo A, Nuñez-Zapata J, Lumbsch HT, Grewe F. Reference-Based Restriction-Site-Associated DNA Sequencing Data Are Useful for Species Delineation in a Recently Diverged Asexually Reproducing Species Complex (Parmeliaceae, Ascomycota). JoF. 2023;9(12):1180. 10.3390/jof9121180 https://hdl.handle.net/20.500.14352/104607 2309-608X https://doi.org/10.3390/jof9121180 Cryptic species are common in lichen-forming fungi and have been reported from different genera in the most speciose family, Parmeliaceae. Herein, we address species delimitation in a group of mainly asexually reproducing Parmelina species. The morphologically distinct P. pastillifera was previously found nested within a morphologically circumscribed P. tiliacea based on several loci. However, these studies demonstrated a relatively high genetic diversity within P. tiliacea sensu lato. Here, we revisit the species delimitation in the group by analyzing single-nucleotide polymorphisms (SNPs) through genome-wide assessment using Restriction-Site-Associated sequencing and population genomic methods. Our data support previous studies and provide further insight into the phylogenetic relationships of the four clades found within the complex. Based on the evidence suggesting a lack of gene flow among the clades, we recognize the four clades as distinct species, P. pastillifera and P. tiliacea sensu stricto, and two new species, P. clandestina sp. nov. and P. mediterranea sp. nov. eng http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 International Reference-based restriction-site-associated DNA sequencing data are useful for species delineation in a recently diverged asexually reproducing species complex (Parmeliaceae, Ascomycota) journal article