2026-06-30T03:13:25Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1056212024-07-04T23:50:09Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author García García, Rosa María author García Rebollar, Pilar author Arias Álvarez, María author Sakr, Osama G. author Bermejo Álvarez, Pablo author Brecchia, G. author Gutiérrez Adán, Alfonso author Zerani, M. author Boiti, C. author Lorenzo González, Pedro Luis 2024-07-04T11:35:33Z 2024-07-04T11:35:33Z 2011-06-21 García-García R. M., Rebollar P. G., Arias-Álvarez M., Sakr O. G., Bermejo-Álvarez P., Brecchia G., Gutierrez-Adan A., Zerani M., Boiti C., Lorenzo P. L. (2011) Acute fasting before conception affects metabolic and endocrine status without impacting follicle and oocyte development and embryo gene expression in the rabbit . Reproduction, Fertility and Development 23, 759-768. https://doi.org/10.1071/RD10298 1031-3613 10.1071/RD10298 https://hdl.handle.net/20.500.14352/105621 1448-5990 https://doi.org/10.1071/RD10298 Food deprivation affects female reproduction. The goal of the present study was to elucidate in the rabbit model the effects of acute energy restriction on ovarian function (follicle development, atresia rate and in vitro oocyte maturation) and embryonic development and gene expression of some candidate genes. Serum metabolic parameters (non-esterified fatty acids (NEFA), triglycerides, glucose, insulin and leptin concentrations) and endocrine markers (oestradiol-17β and progesterone concentrations) were also studied. A control group of nulliparous does fed ad libitum and a 72-h fasted group were used. At the end of the nutritional treatment, the ovaries of half of the animals were retrieved while the other animals were re-fed and artificially inseminated to recover embryos at 84 h after insemination, during the luteal phase. At the end of fasting, increased serum NEFA and decreased leptin concentrations were observed in the fasted group, but no differences appeared in serum steroid concentrations, follicle population and atresia rate or nuclear and cytoplasmic oocyte maturation. In the luteal phase, insulin concentrations increased notably in the fasted group. The number of recovered embryos per female and the speed of embryo development were reduced in the food-deprived group. Acute fasting altered both metabolic and endocrine markers and embryo development, but follicle and oocyte development and embryo gene expression were not affected. eng Attribution-NonCommercial-NoDerivatives 4.0 International Acute fasting before conception affects metabolic and endocrine status without impacting follicle and oocyte development and embryo gene expression in the rabbit journal article 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URL https://docta.ucm.es/bitstreams/d74b54ab-42ba-4a0b-80af-f8da32a60805/download File MD5 22761b174a1270ace1c92782e259b95a 148384 application/pdf Garcia-Garcia.RFD.2011.Ayuno.pdf