2026-06-01T02:36:22Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1056742025-03-18T14:53:45Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Domingo-Diez, Javier author Souiade, Lilia author Manzaneda González, Vanesa author Sánchez-Díez, Marta author Megias, Diego author Guerrero Martínez, Andrés author Ramírez-Castillejo, Carmen author Serrano-Olmedo, Javier author Ramos-Gómez, Milagros 2024-07-05T08:09:06Z 2024-07-05T08:09:06Z 2023-08-27 Domingo-Diez, J.; Souiade, L.; Manzaneda-González, V.; Sánchez-Díez, M.; Megias, D.; Guerrero-Martínez, A.; Ramírez-Castillejo, C.; Serrano-Olmedo, J.; Ramos-Gómez, M. Effectiveness of Gold Nanorods of Different Sizes in Photothermal Therapy to Eliminate Melanoma and Glioblastoma Cells. Int. J. Mol. Sci. 2023, 24, 13306. https://doi.org/10.3390/ijms241713306 1422-0067 10.3390/ijms241713306 https://hdl.handle.net/20.500.14352/105674 https://doi.org/10.3390/ijms241713306 https://www.mdpi.com/1422-0067/24/17/13306 Gold nanorods are the most commonly used nanoparticles in photothermal therapy for cancer treatment due to their high efficiency in converting light into heat. This study aimed to investigate the efficacy of gold nanorods of different sizes (large and small) in eliminating two types of cancer cell: melanoma and glioblastoma cells. After establishing the optimal concentration of nanoparticles and determining the appropriate time and power of laser irradiation, photothermal therapy was applied to melanoma and glioblastoma cells, resulting in the highly efficient elimination of both cell types. The efficiency of the PTT was evaluated using several methods, including biochemical analysis, fluorescence microscopy, and flow cytometry. The dehydrogenase activity, as well as calcein-propidium iodide and Annexin V staining, were employed to determine the cell viability and the type of cell death triggered by the PTT. The melanoma cells exhibited greater resistance to photothermal therapy, but this resistance was overcome by irradiating cells at physiological temperatures. Our findings revealed that the predominant cell-death pathway activated by the photothermal therapy mediated by gold nanorods was apoptosis. This is advantageous as the presence of apoptotic cells can stimulate antitumoral immunity in vivo. Considering the high efficacy of these gold nanorods in photothermal therapy, large nanoparticles could be useful for biofunctionalization purposes. Large nanorods offer a greater surface area for attaching biomolecules, thereby promoting high sensitivity and specificity in recognizing target cancer cells. Additionally, large nanoparticles could also be beneficial for theranostic applications, involving both therapy and diagnosis, due to their superior detection sensitivity. eng Attribution 4.0 International Effectiveness of Gold Nanorods of Different Sizes in Photothermal Therapy to Eliminate Melanoma and Glioblastoma Cells journal article 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