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oai:docta.ucm.es:20.500.14352/1057102025-03-18T15:09:15Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Quinchia, Jennifer author Blázquez-García, Marina author Torrente Rodríguez, Rebeca Magnolia author Ruiz Valdepeñas Montiel, Víctor author Serafín González-Carrato, Verónica author Rejas-González, Raquel author Montero-Calle, Ana author Orozco, Jahir author Pingarrón Carrazón, José Manuel author Barderas Manchado, Rodrigo author Campuzano Ruiz, Susana author 2024-01 Detecting overexpression of cancer biomarkers is an excellent tool for diagnostic/prognostic and follow-up of patients with cancer or their response to treatment. This work illustrates the relevance of interrogating the levels of T-cell immunoglobulin and mucin domain 1 (TIM-1) protein as a diagnostic/prognostic biomarker of high-prevalence breast and lung cancers by using an amperometric disposable magnetic microparticles-assisted immunoplatform. The developed method integrates the inherent advantages of carboxylic acid-functionalized magnetic beads (HOOC-MBs) as pre-concentrator support and the amperometric transduction at screen-printed carbon electrodes (SPCEs). The immunoplatform involves a sandwich-type immunoassay assembled on HOOC-MBs through the specific capture/labeling of TIM-1 using capture antibodies and horseradish peroxidase (HRP)-conjugated biotinylated detection antibodies as biorecognition elements. The magnetic immunoconjugates were confined onto the working electrode (WE) surface of the SPCEs for amperometric detection using the hydroquinone/hydrogen peroxide/HRP (HQ/H2O2/HRP) redox system. The method allows the selective detection of TIM-1 protein over the 87–7500 pg mL−1 concentration range in only 45 min, with a limit of detection of 26 pg mL−1. The developed bioplatform was successfully applied to the analysis of breast and lung cancer cell extracts, providing the first quantitative results of the target glycoprotein in these types of samples. Jennifer Quinchia, Marina Blázquez-García, Rebeca M. Torrente-Rodríguez, Víctor Ruiz-Valdepeñas Montiel, Verónica Serafín, Raquel Rejas-González, Ana Montero-Calle, Jahir Orozco, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano, Disposable electrochemical immunoplatform to shed light on the role of the multifunctional glycoprotein TIM-1 in cancer cells invasion, Talanta, Volume 267, 2024, 125155, ISSN 0039-9140, 0039-9140 10.1016/j.talanta.2023.125155 https://hdl.handle.net/20.500.14352/105710 https://doi.org/10.1016/j.talanta.2023.125155 https://www.sciencedirect.com/science/article/pii/S0039914023009062?via%3Dihub Disposable electrochemical immunoplatform to shed light on the role of the multifunctional glycoprotein TIM-1 in cancer cells invasion