2026-08-22T00:36:59Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1058942025-03-18T12:24:33Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Castejón Ferrer, David author Segura Plaza, José Francisco author Cruz Díaz, Karen Paola author Remiro Yagüe, Víctor author Fernández Valle, María Encarnación author Romero De Ávila Hidalgo, María Dolores author Villa Valverde, Palmira author Cambero Rodríguez, María Isabel 2024-07-10T08:49:50Z 2024-07-10T08:49:50Z 2024-04-13 Castejón, D.; Segura, J.; Cruz-Díaz, K.P.; Remiro, V.; Fernández-Valle, M.E.; Romero de Ávila, M.D.; Villa, P.; Cambero, M.I. A Metabolomics Study by 1H HRMAS NMR: From Sheep Milk to a PressedCurd Cheese: A Proof of Concept. Analytica 2024, 5, 170–186. https:// doi.org/10.3390/analytica5020011 10.3390/analytica5020011 https://hdl.handle.net/20.500.14352/105894 2673-4532 https://doi.org/10.3390/analytica5020011 For the first time, High-Resolution Magic Angle Nuclear Magnetic Resonance spectroscopy (NMR-HRMAS) was applied to directly identify specific metabolites from a Spanish raw ewe’s milk and enzymatic coagulation pressed-curd cheese (Protected Geographical Indication: Castellano) manufactured by two procedures (traditional/artisanal vs. industrial) and including the ewe’s raw milk. The NMR parameters were optimized to study the complex matrixes of this type of cheese. In addition, conventional overcrowded 1H-NMR-HRMAS spectra were selectively simplified by a Carr–Purcell–Meiboom–Gill (CPMG) sequence or a stimulated echo pulse sequence by bipolar gradients (DIFF), thus modulating spin–spin relaxation times and diffusion of molecular components, respectively. 1H-NMR-HRMAS spectroscopy displayed important information about cheese metabolites, which can be associated with different manufacturing processes (industrial vs. traditional) and ripening times (from 2 to 90 days). These results support that this spectroscopy is a useful technique to monitor the ripening process, from raw milk to commercial ripened cheese, using a minimum intact sample, implying the absence of time-consuming sample pretreatments. eng Attribution 4.0 International A Metabolomics Study by 1H HRMAS NMR: From Sheep Milk to a Pressed-Curd Cheese: A Proof of Concept journal article 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