2026-08-22T00:39:43Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1058942025-03-18T12:24:33Zcom_20.500.14352_14col_20.500.14352_15

A Metabolomics Study by 1H HRMAS NMR: From Sheep Milk to a Pressed-Curd Cheese: A Proof of Concept Castejón Ferrer, David Segura Plaza, José Francisco Cruz Díaz, Karen Paola Remiro Yagüe, Víctor Fernández Valle, María Encarnación Romero De Ávila Hidalgo, María Dolores Villa Valverde, Palmira Cambero Rodríguez, María Isabel 641 Foodomics Metabolomics HRMAS-NMR Castellano cheese Fatty acid Amino acid Tecnología de los alimentos 3309 Tecnología de Los Alimentos This article belongs to the Collection "Analytical and Applied Chemistry: the challenges and opportunities for growth in the 21st century" Autorizado por la editorial: portada, índice y primeras páginas de la obra. For the first time, High-Resolution Magic Angle Nuclear Magnetic Resonance spectroscopy (NMR-HRMAS) was applied to directly identify specific metabolites from a Spanish raw ewe’s milk and enzymatic coagulation pressed-curd cheese (Protected Geographical Indication: Castellano) manufactured by two procedures (traditional/artisanal vs. industrial) and including the ewe’s raw milk. The NMR parameters were optimized to study the complex matrixes of this type of cheese. In addition, conventional overcrowded 1H-NMR-HRMAS spectra were selectively simplified by a Carr–Purcell–Meiboom–Gill (CPMG) sequence or a stimulated echo pulse sequence by bipolar gradients (DIFF), thus modulating spin–spin relaxation times and diffusion of molecular components, respectively. 1H-NMR-HRMAS spectroscopy displayed important information about cheese metabolites, which can be associated with different manufacturing processes (industrial vs. traditional) and ripening times (from 2 to 90 days). These results support that this spectroscopy is a useful technique to monitor the ripening process, from raw milk to commercial ripened cheese, using a minimum intact sample, implying the absence of time-consuming sample pretreatments. Ministerio de Ciencia, Innovación y Universidades (España) Sección Deptal. de Farmacia Galénica y Tecnología Alimentaria (Veterinaria) Fac. de Veterinaria TRUE pub 2024-07-10T08:49:50Z 2024-07-10T08:49:50Z 2024-04-13 journal article VoR https://hdl.handle.net/20.500.14352/105894 XXXX-XXXX 10.3390/analytica5020011 2673-4532 eng info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107542RB-C22/ES/METODOLOGIAS DE RESONANCIA MAGNETICA NUCLEAR (RMN) COMO HERRAMIENTAS DE AUTOMATIZACION Y CONTROL EN EL SECTOR CARNICO/ Castejón, D.; Segura, J.; Cruz-Díaz, K.P.; Remiro, V.; Fernández-Valle, M.E.; Romero de Ávila, M.D.; Villa, P.; Cambero, M.I. A Metabolomics Study by 1H HRMAS NMR: From Sheep Milk to a PressedCurd Cheese: A Proof of Concept. Analytica 2024, 5, 170–186. https:// doi.org/10.3390/analytica5020011 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ open access application/pdf MDPI