2026-06-29T02:29:27Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1068202025-09-18T14:43:04Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Gil-Gil, Teresa author Cuesta, Trinidad author Hernando-Amado, Sara author Reales-Calderón, Jose Antonio author Corona, Fernando author Linares, Juan F. author Martínez, José L. author 2023 Pseudomonas aeruginosa is a ubiquitous nosocomial opportunistic pathogen that harbors many virulence determinants. Part of P. aeruginosa success colonizing a variety of habitats resides in its metabolic robustness and plasticity, which are the basis of its capability of adaptation to different nutrient sources and ecological conditions, including the infected host. Given this situation, it is conceivable that P. aeruginosa virulence might be, at least in part, under metabolic control, in such a way that virulence determinants are produced just when needed. Indeed, it has been shown that the catabolite repression control protein Crc, which together with the RNA chaperon Hfq regulates the P. aeruginosa utilization of carbon sources at the post-transcriptional level, also regulates, directly or indirectly, virulence-related processes in P. aeruginosa. Among them, Crc regulates P. aeruginosa cytotoxicity, likely by modulating the activity of the Type III Secretion System (T3SS), which directly injects toxins into eukaryotic host cells. The present work shows that the lack of Crc produces a Type III Secretion-defective phenotype in P. aeruginosa. The observed impairment is a consequence of a reduced expression of the genes encoding the T3SS, together with an impaired secretion of the proteins involved. Our results support that the impaired T3SS activity of the crc defective mutant is, at least partly, a consequence of a defective protein export, probably due to a reduced proton motive force. This work provides new information about the complex regulation of the expression and the activity of the T3SS in P. aeruginosa. Our results highlight the need of a robust bacterial metabolism, which is defective in the ∆crc mutant, to elicit complex and energetically costly virulence strategies, as that provided by the T3SS. Gil-Gil, T.; Cuesta, T.; Hernando-Amado, S.; Reales-Calderón, J.A.; Corona, F.; Linares, J.F.; Martínez, J.L. Virulence and Metabolism Crosstalk: Impaired Activity of the Type Three Secretion System (T3SS) in a Pseudomonas aeruginosa Crc-Defective Mutant. Int. J. Mol. Sci. 2023, 24, 12304. https://doi.org/10.3390/ijms241512304 1422-0067 10.3390/ijms241512304 https://hdl.handle.net/20.500.14352/106820 https://doi.org/10.3390/ijms241512304 Virulence and Metabolism Crosstalk: Impaired Activity of the Type Three Secretion System (T3SS) in a Pseudomonas aeruginosa Crc-Defective Mutant