2026-06-07T16:09:16Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1073612024-08-02T23:46:21Zcom_20.500.14352_14col_20.500.14352_15

An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus Cea Callejo, Pablo Arca Lafuente, Sonia Gómez-Lucía Duato, María Esperanza Domenech Gómez, Ana María Biarnés, Mar Blanco, Ángela Benítez Rico, Laura Madrid González, Ricardo Airborne animal viral pathogens can rapidly spread and become a global threat, resulting in substantial socioeconomic and health consequences. To prevent and control potential epidemic outbreaks, accurate, fast, and affordable point-of-care (POC) tests are essential. As a proof-of-concept, we have developed a molecular system based on the loop-mediated isothermal amplification (LAMP) technique for avian metapneumovirus (aMPV) detection, an airborne communicable agent mainly infecting turkeys and chickens. For this purpose, a colorimetric system was obtained by coupling the LAMP technique with specific DNA-functionalized AuNPs (gold nanoparticles). The system was validated using 50 different samples (pharyngeal swabs and tracheal tissue) collected from aMPV-infected and non-infected chickens and turkeys. Viral detection can be achieved in about 60 min with the naked eye, with 100% specificity and 87.88% sensitivity for aMPV. In summary, this novel molecular detection system allows suitable virus testing in the field, with accuracy and limit of detection (LOD) values highly close to qRT-PCR-based diagnosis. Furthermore, this system can be easily scalable to a platform for the detection of other viruses, addressing the current gap in the availability of POC tests for viral detection in poultry farming. 2024-08-02T11:03:18Z 2024-08-02T11:03:18Z 2024-07-10 journal article Cea-Callejo, P., Arca-Lafuente, S., Gomez-Lucia, E. et al. An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus. Appl Microbiol Biotechnol 108, 414 (2024). https://doi.org/10.1007/s00253-024-13243-x 0175-7598 10.1007/s00253-024-13243-x https://hdl.handle.net/20.500.14352/107361 1432-0614 https://doi.org/10.1007/s00253-024-13243-x 38985204 eng PID2020-114956GB-100 IND2017/BMD-7683 IND2019/BIO-17124 PAIT08/21–02/2021–08 CT19/23-INVM-19 http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 International Springer