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oai:docta.ucm.es:20.500.14352/1073882025-09-02T15:47:33Zcom_20.500.14352_14col_20.500.14352_15

Check-control of inflammation displayed by bone marrow mesenchymal stem cells in rheumatoid arthritis patients Lamas, José Ramón Mucientes, Arkaitz Lajas Petisco, Cristina De Jesús Fernández Gutiérrez, Benjamín Lópiz Morales, María Yaiza Marco Martínez, Fernando Jover Jover, Juan Ángel Abásolo Alcázar, Lydia Rodríguez Rodríguez, Luis Background: Mesenchymal stem cells (MSCs) are a promising treatment of different musculoskeletal diseases including osteoarthritis and rheumatoid arthritis (RA). Results from different approaches in this treatment have been not conclusive. Aim: To analyze factors related to interactions between peripheral blood mononuclear cells (PBMCs) and MSCs and the influence of cellular activation. Materials & methods: PBMCs from RA patients and healthy controls (HC) were obtained. MSCs from bone marrow (BM-MSCs) were obtained from six donors. CD4, CD25, CD69 and CD127 expression was measured by flow cytometry. Repeated measures analysis of variance (ANOVA) models were performed using activation, co-culture with BM-MSCs and time of culture (24 h, 72 h, 6 days) as within-subject variables. Results: PBMCs activated and co-cultured with BM-MSCs showed a lower proportion of CD25-positive and CD25high/CD127low-negative cells in both RA and HC. Additionally, a maintained expression of CD69 was also observed in RA and HC when PBMCs were activated and co-cultured with BM-MSCs. Conclusion: Both PBMC activation grade and RA disease activity influence the immunomodulatory effect of BM-MSCs on T-cell activation. 2024-08-05T11:25:28Z 2024-08-05T11:25:28Z 2024-08-05T11:25:28Z 2019 journal article https://hdl.handle.net/20.500.14352/107388 1750-7448 10.2217/imt-2019-0091 1750-743X eng info:eu-repo/grantAgreement/MINECO//CP12%2F03129/ES/CP12%2F03129/ Lamas JR, Mucientes A, Lajas C, Fernández-Gutiérrez B, Lópiz Y, Marco F, Jover JA, Abásolo L, Rodriguez-Rodriguez L. Check-control of inflammation displayed by bone marrow mesenchymal stem cells in rheumatoid arthritis patients. Immunotherapy. 2019 Sep;11(13):1107-1116. doi: 10.2217/imt-2019-0091. Epub 2019 Aug 5. PMID: 31378114. http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 International Taylor & Francis