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oai:docta.ucm.es:20.500.14352/1074032025-03-18T14:33:08Zcom_20.500.14352_14col_20.500.14352_15

Camila, Arvinius Civantos, Ana Rodríguez Bobada, Cruz Rojo, Francisco Javier Pérez Gallego, Daniel Lópiz Morales, María Yaiza Marco Martínez, Fernando 2024-08-06T11:47:38Z 2024-08-06T11:47:38Z 2020-11-09 Arvinius C, Civantos A, Rodríguez-Bobada C, Rojo FJ, Pérez-Gallego D, Lopiz Y, Marco F. Enhancement of in vivo supraspinatus tendon-to-bone healing with an alginate-chitin scaffold and rhBMP-2. Injury. 2021 Jan;52(1):78-84. doi: 10.1016/j.injury.2020.11.019. Epub 2020 Nov 9. PMID: 33223258. 1879-0267 10.1016/j.injury.2020.11.019 https://hdl.handle.net/20.500.14352/107403 0020-1383 https://doi.org/10.1016/j.injury.2020.11.019 33223258 https://www.journals.elsevier.com/injury Introduction: Rotator cuff disorders present a high retear rate despite advances in surgical treatment. Tissue engineering could therefore be interesting in order to try to enhance a more biological repair. RhBMP-2 is one of the most osteogenic growth factors and it also induces the formation of collagen type I. However, it has a short half-life and in order to get a more stable release over time it could be integrated in a more slowly degradable carrier, such as an alginate-chitin scaffold. The aim of this study was to investigate the role of the alginate-chitin scaffold alone and in combination with different concentrations of rhBMP-2 when applied on chronic rotator cuff lesions in a rat model. Materials and methods: We performed an experimental study with 80 Sprague-Dawley rats, 8 months old, with a chronic rupture of the supraspinatus tendon that was repaired with a modified Mason Allen suture. A scaffold was applied over the suture and 4 groups were obtained; suture (S) only suture, double control (DC) alginate and chitin scaffold, single sample (SS) scaffold of alginate with rhBMP-2 (20 µg rhBMP-2) and chitin, double sample (DS) a scaffold containing alginate with rhBMP-2 and chitin with rhBMP-2 (40 µg rhBMP-2). Macroscopic, histological and biomechanical studies were performed at 4 months after reparation. Results: The modified Åström and Rausing's histological scale (the higher the score the worse outcome, 0 points=native tendon) was applied: S got 52 points compared to DC 30 (p = 0,034), SS 22 (p = 0,009) and DS 16 (p = 0,010). Biomechanically the maximum load was highest in DC (63,05 N), followed by DS (61,60 N), SS (52,35 N) and S (51,08), p = 0,025 DS vs S. As to the elastic constant a higher value was obtained in DC (16,65), DS (12,55) and SS (12,20) compared to S (9,33), p = 0,009 DC vs S and 0,034 DS vs S. Conclusions: The alginate-chitin scaffold seems to promote a more biological response after the reparation of a chronic rotator cuff lesion. Its effect is further enhanced by the addition of rhBMP-2 since the osteotendinous junction is more native-like and has better biomechanical properties. eng restricted access Enhancement of in vivo supraspinatus tendon–to-bone healing with an alginate-chitin scaffold and rhBMP-2 journal article