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00925njm 22002777a 4500 dc Marquina Díaz, Domingo author Barroso, J. author Santos de la Sen, Antonio author Martínez Peinado, José author 2001 The optimal conditions for the production of the killer toxin of Debaryomyces hansenii CYC 1021 have been studied. The lethal activity of the killer toxin increased with the presence of NaCl in the medium used for testing the killing action. Production of the killer toxin was stimulated in the presence of proteins of complex culture media. Addition of nonionic detergents and other additives, such as dimethylsulfoxide enhanced killer toxin production significantly. Killer toxin secretion pattern followed the growth curve and reached its maximum activity at the early stationary phase. Optimal stability was observed at pH 4.5 and temperatures up to 20 °C. Above pH 4.5 a steep decrease of the stability was noted. The activity was hardly detectable at pH 5.1. Marquina, D., et al. «Production and Characteristics of Debaryomyces Hansenii Killer Toxin». Microbiological Research, vol. 156, n.o 4, 2001, pp. 387-91. DOI.org (Crossref), https://doi.org/10.1078/0944-5013-00117. 0944-5013 10.1078/0944-5013-00117 https://hdl.handle.net/20.500.14352/108224 1618-0623 https://doi.org/10.1078/0944-5013-00117 11770858 https://www.sciencedirect.com/science/article/pii/S094450130470052X?via%3Dihub Production and characteristics of debaryomyces hansenii killer toxin