2026-07-19T12:24:47Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1084532025-03-18T13:22:51Zcom_20.500.14352_14col_20.500.14352_15

Herrero-Fernandez, Beatriz Gómez Bris, Raquel Ortega-Zapero, Marina Saez, Angela Iborra Martín, Salvador Zorita, Virginia Quintas, Ana Vazquez, Enrique Dopazo, Ana Sánchez Madrid, Francisco Arribas, Silvia Magdalena González Granado, José María 2024-09-30T07:09:33Z 2024-09-30T07:09:33Z 2024-09-12 Herrero-Fernández B, Ortega-Zapero M, Gómez-Bris R, et al. Role of lamin A/C on dendritic cell function in antiviral immunity. Cell Mol Life Sci. 2024;81(1):400. Published 2024 Sep 12. doi:10.1007/s00018-024-05423-9 10.1007/s00018-024-05423-9 https://hdl.handle.net/20.500.14352/108453 https://doi.org/10.1007/s00018-024-05423-9 https://link.springer.com/article/10.1007/s00018-024-05423-9 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11393282/ Dendritic cells (DCs) play a crucial role in orchestrating immune responses, particularly in promoting IFNγ-producing-CD8 cytotoxic T lymphocytes (CTLs) and IFNγ-producing-CD4 T helper 1 (Th1) cells, which are essential for defending against viral infections. Additionally, the nuclear envelope protein lamin A/C has been implicated in T cell immunity. Nevertheless, the intricate interplay between innate and adaptive immunity in response to viral infections, particularly the role of lamin A/C in DC functions within this context, remains poorly understood. In this study, we demonstrate that mice lacking lamin A/C in myeloid LysM promoter-expressing cells exhibit a reduced capacity to induce Th1 and CD8 CTL responses, leading to impaired clearance of acute primary Vaccinia virus (VACV) infection. Remarkably, in vitro-generated granulocyte macrophage colony-stimulating factor bone marrow-derived DCs (GM-CSF BMDCs) show high levels of lamin A/C. Lamin A/C absence on GM-CSF BMDCs does not affect the expression of costimulatory molecules on the cell membrane but it reduces the cellular ability to form immunological synapses with naïve CD4 T cells. Lamin A/C deletion induces alterations in NFκB nuclear localization, thereby influencing NF-κB-dependent transcription. Furthermore, lamin A/C ablation modifies the gene accessibility of BMDCs, predisposing these cells to mount a less effective antiviral response upon TLR stimulation. This study highlights the critical role of DCs in interacting with CD4 T cells during antiviral responses and proposes some mechanisms through which lamin A/C may modulate DC function via gene accessibility and transcriptional regulation eng http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 International Role of Lamin A/C on dendritic cell function in antiviral immunity journal article