2026-07-31T10:11:46Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1087282025-03-18T13:01:50Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Campanero, Cristina author Dzung B. Diep author Arbulu, Sara author Del Campo, Rosa author Ingolf F. Nes author Cintas Izarra, Luis Miguel author Muñoz Atienza, Estefanía author Feito Hermida, Javier author Hernández Cruza, Pablo Elpidio author Herranz Sorribes, Carmen editor Anirudh K. Singh 2024-10-07T14:53:44Z 2024-10-07T14:53:44Z 2020-03 Campanero, C., Muñoz-Atienza, E., Diep, D. B., Feito, J., Arbulu, S., Del Campo, R., Nes, I. F., Hernández, P. E., Herranz, C., & Cintas, L. M. (2020). Biochemical, genetic and transcriptional characterization of multibacteriocin production by the anti-pneumococcal dairy strain Streptococcus infantarius LP90. PLOS ONE, 15(3), e0229417. https://doi.org/10.1371/journal.pone.0229417 1932-6203 10.1371/journal.pone.0229417 https://hdl.handle.net/20.500.14352/108728 https://doi.org/10.1371/journal.pone.0229417 32134941 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229417 Streptococcus pneumoniae infections are one of the major causes of morbility and mortality worldwide. Although vaccination and antibiotherapy constitute fundamental and complementary strategies against pneumococcal infections, they present some limitations including the increase in non-vaccine serotypes and the emergence of multidrug-resistances, respectively. Ribosomally-synthesized antimicrobial peptides (i.e. bacteriocins) produced by Lactic Acid Bacteria (LAB) may represent an alternative or complementary strategy to antibiotics for the control of pneumococal infections. We tested the antimicrobial activity of 37 bacteriocinogenic LAB, isolated from food and other sources, against clinical S. pneumoniae strains. Streptococcus infantarius subsp. infantarius LP90, isolated from Venezuelan water-buffalo milk, was selected because of its broad and strong anti-pneumococcal spectrum. The in vitro safety assessment of S. infantarius LP90 revealed that it may be considered avirulent. The analysis of a 19,539-bp cluster showed the presence of 29 putative open reading frames (ORFs), including the genes encoding 8 new class II-bacteriocins, as well as the proteins involved in their secretion, immunity and regulation. Transcriptional analyses evidenced that the induction factor (IF) structural gene, the bacteriocin/IF transporter genes, the bacteriocin structural genes and most of the bacteriocin immunity genes were transcribed. MALDI-TOF analyses of peptides purified using different multichromatographic procedures revealed that the dairy strain S. infantarius LP90 produces at least 6 bacteriocins, including infantaricin A1, a novel anti-pneumococcal two-peptide bacteriocin. eng Attribution-NonCommercial-NoDerivatives 4.0 International Biochemical, genetic and transcriptional characterization of multibacteriocin production by the anti-pneumococcal dairy strain Streptococcus infantarius LP90 journal article 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URL https://docta.ucm.es/bitstreams/4105ad7e-a67b-4e43-b2df-57bb1e66bc82/download File MD5 1efa5637d132497fae9f25a92681573d 1428775 application/pdf Campanero et al 2020 PlosOne.pdf URL https://docta.ucm.es/bitstreams/d1552453-b528-45c1-a1a5-ac1ddddcad0a/download File MD5 1daa85392d0b1cae788c72e2c0e23c29 69988 text/plain Campanero et al 2020 PlosOne.pdf.txt