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Biochemical, genetic and transcriptional characterization of multibacteriocin production by the anti-pneumococcal dairy strain Streptococcus infantarius LP90 Campanero, Cristina Dzung B. Diep Arbulu, Sara Del Campo, Rosa Ingolf F. Nes Cintas Izarra, Luis Miguel Muñoz Atienza, Estefanía Feito Hermida, Javier Hernández Cruza, Pablo Elpidio Herranz Sorribes, Carmen Anirudh K. Singh 579.62 Lactic Acid Bacteria Streptococcus infantarius Bacteriocin Infantaricins Dairy origin Microbiología (Veterinaria) 2414 Microbiología Streptococcus pneumoniae infections are one of the major causes of morbility and mortality worldwide. Although vaccination and antibiotherapy constitute fundamental and complementary strategies against pneumococcal infections, they present some limitations including the increase in non-vaccine serotypes and the emergence of multidrug-resistances, respectively. Ribosomally-synthesized antimicrobial peptides (i.e. bacteriocins) produced by Lactic Acid Bacteria (LAB) may represent an alternative or complementary strategy to antibiotics for the control of pneumococal infections. We tested the antimicrobial activity of 37 bacteriocinogenic LAB, isolated from food and other sources, against clinical S. pneumoniae strains. Streptococcus infantarius subsp. infantarius LP90, isolated from Venezuelan water-buffalo milk, was selected because of its broad and strong anti-pneumococcal spectrum. The in vitro safety assessment of S. infantarius LP90 revealed that it may be considered avirulent. The analysis of a 19,539-bp cluster showed the presence of 29 putative open reading frames (ORFs), including the genes encoding 8 new class II-bacteriocins, as well as the proteins involved in their secretion, immunity and regulation. Transcriptional analyses evidenced that the induction factor (IF) structural gene, the bacteriocin/IF transporter genes, the bacteriocin structural genes and most of the bacteriocin immunity genes were transcribed. MALDI-TOF analyses of peptides purified using different multichromatographic procedures revealed that the dairy strain S. infantarius LP90 produces at least 6 bacteriocins, including infantaricin A1, a novel anti-pneumococcal two-peptide bacteriocin. Ministerio de Ciencia, Innovación y Universidades (España) Ministerio de Economía y Competitividad (España) Comunidad de Madrid Junta de Galicia Universidad Complutense de Madrid Sección Dptal. de Nutrición y Ciencia de los Alimentos (Veterinaria) Fac. de Veterinaria TRUE pub 2024-10-07T14:53:44Z 2024-10-07T14:53:44Z 2020-03 journal article VoR https://hdl.handle.net/20.500.14352/108728 1932-6203 10.1371/journal.pone.0229417 eng info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094907-B-I00/ES/DISEÑO DE BACTERIAS LACTICAS (BAL) PRODUCTORAS DE BACTERIOCINAS Y EVALUACION DE BAL, PRODUCTORAS Y NO PRODUCTORAS DE BACTERIOCINAS, COMO PROBIOTICOS PARA LA ACUICULTURA/ info:eu-repo/grantAgreement/MINECO//AGL2012-34829/ES/DISEÑO DE BACTERIAS LACTICAS (BAL) Y LEVADURAS COMO FACTORIAS CELULARES DE PRODUCCION DE PEPTIDOS HIBRIDOS ANTIMICROBIANOS Y DE OTROS PEPTIDOS BIOACTIVOS/ info:eu-repo/grantAgreement/Comunidad de Madrid/S-2009/AGR-1489 info:eu-repo/grantAgreement/Comunidad de Madrid/S-2013/ABI- 2747 info:eu-repo/grantAgreement/Junta de Galicia/07CSA007E Campanero, C., Muñoz-Atienza, E., Diep, D. B., Feito, J., Arbulu, S., Del Campo, R., Nes, I. F., Hernández, P. E., Herranz, C., & Cintas, L. M. (2020). Biochemical, genetic and transcriptional characterization of multibacteriocin production by the anti-pneumococcal dairy strain Streptococcus infantarius LP90. PLOS ONE, 15(3), e0229417. https://doi.org/10.1371/journal.pone.0229417 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ open access application/pdf Public Library of Science (PLOS)