2026-06-08T14:41:23Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1089862024-10-15T23:53:18Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Pérez Montero, María Eugenia author Fermín Rodríguez, María Luisa author Portero Fuentes, Miriam author Sarquis, J. author Juan Ferré, Lucía De author Miró Corrales, Guadalupe author Cruz López, Fátima author Illera Del Portal, Juan Carlos author Cáceres Ramos, Sara Cristina author 2024 Background: Total antioxidant status (TAS) is one of the most widely used oxidative stress biomarkers, but the lack of canine RI and the influence of analytical factors hinder its application in clinical practice. Objectives: The aims of this study were to establish canine assay-specific RI for TAS and evaluate the sources of biological variation and the association between TAS and multiple hematologic and biochemical variables. Methods: Blood samples from 190 clinically healthy dogs were collected, encompassing pet dogs (82), police dogs (56), and shelter dogs (52). After hematologic and biochemical analysis, serum TAS was determined by means of a commercial 2,2'-azinobis (3-ethylbenzthiazolin-6-sulfonic acid) (ABTS) test. The American Society for Veterinary Clinical Pathology guidelines were followed to establish the RI, employing nonparametric methods. Univariate analysis and multivariate analysis were conducted to assess the influence of biological and analytical variables, yielding a final regression model. Results: The final reference population comprised 143 dogs, for which the RI was established (1.41-2.27 mmol/L). Partitioning was applied to the three study groups. The regression model revealed that police dogs had significantly higher TAS values than pet dogs. Furthermore, significant associations between four biochemical variables (albumin, globulins, cholesterol, and aspartate aminotransferase) and serum TAS were found. Conclusions: This is the first study to establish RI for serum TAS in a large and heterogeneous canine population and provide data on its relationship with analytical variables. These findings could potentially improve the interpretation of TAS in clinical environments. Perez-Montero B, Fermin-RodriguezML, Portero-Fuentes M, et al. Serum total antioxidant statusin dogs: Reference intervals and influence of multiplebiological and analytical factors. Vet Clin Pathol. 2024;00:1-10. doi:10.1111/vcp.13381 0275-6382 10.1111/vcp.13381 https://hdl.handle.net/20.500.14352/108986 1939-165X http://doi.org/10.1111/vcp.13381 39396933 Serum total antioxidant status in dogs: Reference intervals and influence of multiple biological and analytical factors