2026-06-27T10:33:03Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1090332024-10-17T00:03:40Zcom_20.500.14352_14col_20.500.14352_15

Rivero, Cintia Wanda García, Natalia Soledad Fernández Lucas, Jesús Betancor, Lorena Romanelli, Gustavo Pablo Trelles, Jorge Abel 2024-10-16T14:38:42Z 2024-10-16T14:38:42Z 2021 Rivero CW, García NS, Fernández-Lucas J, Betancor L, Romanelli GP, Trelles JA. Green Production of Cladribine by Using Immobilized 2′-Deoxyribosyltransferase from Lactobacillus delbrueckii Stabilized through a Double Covalent/Entrapment Technology. Biomolecules 2021;11:657. https://doi.org/10.3390/biom11050657 10.3390/biom11050657 https://hdl.handle.net/20.500.14352/109033 2218-273X https://doi.org/10.3390/biom11050657 https://www.mdpi.com/2218-273X/11/5/657 Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60 °C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale. eng http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 International Green production of cladribine by using immobilized 2'-deoxyribosyltransferase from Lactobacillus delbrueckii stabilized through a double covalent/entrapment Technology journal article