2026-06-29T03:13:09Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1091442025-03-18T13:47:40Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Linillos Pradillo, Beatriz author Paredes Royano, Sergio Damián author Ortiz-Cabello, María author Schlumpf, Margret author Lichtensteiger, Walter author Vara Ameigeiras, Elena María author Fernández-Tresguerres Hernández, Jesús Ángel author Rancán, Lisa 2024-10-21T11:05:06Z 2024-10-21T11:05:06Z 2023 Linillos-Pradillo, B.; Paredes, S.D.; Ortiz-Cabello, M.; Schlumpf, M.; Lichtensteiger, W.; Vara, E.; Tresguerres, J.A.F.; Rancan, L. Activation of NLRP3 Inflammasome in Liver of Long Evans Lactating Rats and Its Perinatal Effects in the Offspring after Bisphenol F Exposure. Int. J. Mol. Sci. 2023, 24, 14129. https://doi.org/ 10.3390/ijms241814129 1661-6596 10.3390/ijms241814129 https://hdl.handle.net/20.500.14352/109144 https://www.mdpi.com/1422-0067/24/18/14129 The liver is the organ responsible for the metabolism and detoxification of BPF, the BPA analogue that is replacing it in plastic-based products. It is not known whether BPF can trigger inflammatory responses via the NLRP3 inflammasome, which plays a major role in the development of liver disease. The aim of this study was to evaluate nitrosative stress species (RNS) and NLRP3 inflammasome activation in the liver of lactating dams after BPF exposure. Moreover, it was studied whether this effect could also be observed in the liver of female and male offspring at postnatal day 6 (PND6). 36 Long Evans rats were randomly distributed according to oral treatment into three groups: Control, BPF-low dose (LBPF; 0.0365 mg/kg b.w./day) group and BPF-high dose (HBPF; 3.65 mg/kg b.w./day) group. The levels of nitrosative stress-inducing proteins (eNOS, iNOS, HO-1d), NLRP3 inflammasome components (NLRP3, PyCARD, CASP1) and proinflammatory cytokines (IL-1β, IL-18, IFN-γ and TNF-α) were measured by gene and protein expression in the liver of lactating dams and in female and male PND6 offspring. Lactating dams treated with LBPF showed a significant increase in iNOS and HO-1d, activation of NLRP3 components (NLRP3, PyCARD, CASP1) and promoted the release of proinflammatory cytokines such as IL-1β, IL-18, IFN-γ and TNF-α. Similar effects were found in female and male PND6 offspring after perinatal exposure. LBPF oral administration and perinatal exposure caused an increase of nitrosative stress markers and proinflammatory cytokines. Also, NLRP3 inflammasome activation was significantly increased in in the liver of lactating dams and PND6 offspring. eng Attribution-NonCommercial-NoDerivatives 4.0 International Activation of NLRP3 inflammasome in liver of long evans lactating rats and its perinatal effects in the offspring after bisphenol F exposure journal article 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URL https://docta.ucm.es/bitstreams/3f9c8be4-b816-4145-bd2e-75f291185081/download File MD5 763070d9c25c21ff9702d55712941e9e 3554523 application/pdf ijms-24-14129 (1).pdf URL https://docta.ucm.es/bitstreams/5d66fe6d-5079-4135-88ad-5d46f645fa02/download File MD5 c7c5093d3224c43d79a9ddb69fbad68a 64147 text/plain ijms-24-14129 (1).pdf.txt