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Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters Gimeno Martos, Silvia Bosa, Luigia Lorenzo González, Pedro Luis Arias Álvarez, María Castellini, Cesare García Rebollar, Pilar García García, Rosa María 636.082 Acrosome reaction Capacitation NGF Rabbit sperm Sperm motility Producción animal 3104.11 Reproducción β-nerve growth factor (βNGF) plays a crucial role in reproductive physiology and sperm quality. Enzymatic activity of seminal plasma and vaginal fluids reduces available βNGF and it has been demonstrated that chitosan microspheres could protect rrβNGF from degradation. This study examined the effects of microencapsulated rrbNGF with chitosan on rabbit sperm viability, motility and capacitation status. Results showed that 0.5 and 1 μg/mL of microencapsulated rrβNGF, as well as free rrβNGF or empty microspheres, did not adversely affect sperm viability or total motility after 2 h of incubation. However, the highest progressivity kinetic parameters were observed with 1 μg/mL free rrβNGF, while the highest curvilinear velocity (VCL) occurred with 0.5 μg/mL microencapsulated rrβNGF. Empty chitosan microspheres did not induce acrosome reaction (AR), but both concentrations of free and rrβNGFch favoured AR during in vitro incubation. The study suggests that using chitosan spheres did not show any adverse effects on sperm traits, unlike free rβNGF and rrβNGFch promoted capacitation and AR. Further research is needed to explore the potential of rrβNGFch in modifying in vitro capacitation and inducing ovulation during artificial insemination. Ministerio de Economía, Comercio y Empresa (España) European Commission Universidad Complutense de Madrid Depto. de Fisiología Depto. de Producción Animal Fac. de Veterinaria TRUE pub 2024-10-23T17:07:24Z 2024-10-23T17:07:24Z 2024 journal article VoR https://hdl.handle.net/20.500.14352/109346 0936-6768 10.1111/rda.14636 1439-0531 eng info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094404-B-C22/ES/FUNCIONES FISIOLOGICAS DEL BETA NGF RECOMBINANTE DE CONEJO SOBRE LOS OOCITOS Y EMBRIONES IN VIVO E IN VITRO/ 920249 Gimeno-Martos, S., Bosa, L., Lorenzo, P. L., Arias-Alvarez, M., Castellini, C., García-Rebollar, P., & García-García, R. M. (2024). Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters. Reproduction in Domestic Animals, 59(Suppl. 3), e14636. https://doi.org/10.1111/rda.14636 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ open access application/pdf Wiley