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oai:docta.ucm.es:20.500.14352/1097612025-12-19T15:52:48Zcom_20.500.14352_14col_20.500.14352_15

Walnut jug r 1 is responsible for primary sensitization among patients suffering walnut-hazelnut 2S albumin cross-reactivity Castromil Benito, Estela S. Betancor, Diana Parrón Ballesteros, Jorge Bueno Díaz, Cristina Gutiérrez Díaz, Gloria Turnay, Javier de las Heras, Manuel Cuesta Herranz, Javier Villalba, Mayte Pastor Vargas, Carlos 577.1 2S albumins Cor a 14 Jug r 1 Allergen Component-resolved diagnosis Cross-reactivity Food allergy Molecular diagnosis Primary sensitizer Seed storage proteins Food allergy Jug r 1 Cor a 14 Bioquímica (Química) Alergología 2302 Bioquímica Walnut and hazelnut coallergy is a frequent manifestation in clinical practice whose molecular basis remains unclear. For this purpose, walnut-hazelnut cross-reactivity was evaluated in 20 patients allergic to one or both tree nuts and sensitized to their 2S albumins. Immunoblotting assays showed that 85% of patients recognized Jug r 1, walnut 2S albumin, which was associated with the development of severe symptoms; 50% of them corecognized hazelnut 2S albumin, Cor a 14. Both allergens were isolated using chromatographic techniques. Inhibition ELISAs revealed that Jug r 1 strongly inhibited the binding of Cor a 14-specific IgE, but Cor a 14 only partially inhibited Jug r 1-specific IgE binding. Our results showed that patients sensitized to walnut/hazelnut 2S albumins were not a homogeneous population. There were patients sensitized to specific epitopes of walnut 2S albumins and patients sensitized to cross-reactive epitopes between walnut and hazelnut, with Jug r 1 being the primary sensitizer. Ministerio de Ciencia e Innovación (España) Depto. de Bioquímica y Biología Molecular Fac. de Ciencias Químicas TRUE pub 2024-10-30T09:35:06Z 2024-10-30T09:35:06Z 2024-07-31 journal article VoR https://hdl.handle.net/20.500.14352/109761 XXXX-XXXX 10.1021/acs.jafc.4c03603 eng info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-116692RB-I00/ES/ALERGENOS Y EJE INTESTINO-PULMON: NUEVAS APROXIMACIONES AL DIAGNOSTICO Y TRATAMIENTO DE LA ALERGIA/ Castromil-Benito ES, Betancor D, Parrón-Ballesteros J, Bueno-Díaz C, Gutiérrez-Díaz G, Turnay J, Heras ML, Cuesta-Herranz J, Villalba M, Pastor-Vargas C. Walnut Jug r 1 is Responsible for Primary Sensitization among Patients Suffering Walnut-Hazelnut 2S Albumin Cross-Reactivity. J Agric Food Chem. 2024 Aug 14;72(32):18162-18170. doi: 10.1021/acs.jafc.4c03603 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ open access application/pdf ACS Publications