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oai:docta.ucm.es:20.500.14352/1099682025-03-18T13:40:28Zcom_20.500.14352_14col_20.500.14352_15

Multi-omics framework to reveal the molecular determinants of fermentation performance in wine yeast populations De Celis Rodríguez, Miguel Ruiz Ruiz, Javier Benítez Domínguez, Belén Vicente Sánchez, Javier Tomasi, Sandra Izquierdo Gea, Sergio Rozes, Nicolás Ruiz de Villa, Candela Gombau, Jordi Zamora, Fernando Barroso del Jesús, Alicia Terrón Camero, Laura C. Andrés León, Eduardo Santos de la Sen, Antonio Belda Aguilar, Ignacio 579.66 577.15 663.25 Microbiología (Biología) Bioquímica (Biología) Tecnología de los alimentos 3302.03 Microbiología Industrial 3309.92 Bioquímica y Microbiología de Los Procesos Fermentativos 3309.29 Vino 2302.12 Fermentación Background Connecting the composition and function of industrial microbiomes is a major aspiration in microbial biotechnology. Here, we address this question in wine fermentation, a model system where the diversity and functioning of fermenting yeast species are determinant of the flavor and quality of the resulting wines. Results First, we surveyed yeast communities associated with grape musts collected across wine appellations, revealing the importance of environmental (i.e., biogeography) and anthropic factors (i.e., farming system) in shaping community composition and structure. Then, we assayed the fermenting yeast communities in synthetic grape must under common winemaking conditions. The dominating yeast species defines the fermentation performance and metabolite profile of the resulting wines, and it is determined by the initial fungal community composition rather than the imposed fermentation conditions. Yeast dominance also had a more pronounced impact on wine meta-transcriptome than fermentation conditions. We unveiled yeast-specific transcriptomic profiles, leveraging different molecular functioning strategies in wine fermentation environments. We further studied the orthologs responsible for metabolite production, revealing modules associated with the dominance of specific yeast species. This emphasizes the unique contributions of yeast species to wine flavor, here summarized in an array of orthologs that defines the individual contribution of yeast species to wine ecosystem functioning. Conclusions Our study bridges the gap between yeast community composition and wine metabolite production, providing insights to harness diverse yeast functionalities with the final aim to producing tailored high-quality wines. European Commission Ministerio de Ciencia, Innovación y Universidades (España) Universidad Complutense de Madrid Comunidad de Madrid Junta de Andalucía Depto. de Genética, Fisiología y Microbiología Fac. de Ciencias Biológicas TRUE pub CT17/17-CT18/17 CT58/21-CT59/21 09-PIN1-00009.8/2022 FPU21/06830 POSTDOC_20 _00541 POSTDOC_21 _00394 2024-11-04T19:30:42Z 2024-11-04T19:30:42Z 2024-10 journal article VoR https://hdl.handle.net/20.500.14352/109968 XXXX-XXXX 10.1186/s40168-024-01930-w 2049-2618 eng info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105834GA-I00/ES/ECOLOGIA FUNCIONAL Y BIOLOGIA DE SISTEMAS PARA LA MODELIZACION DEL PROCESO DE FERMENTACION VINICA/ info:eu-repo/grantAgreement/MICIU/AEI/ 10.13039/501100011033//PID2022-138343NB-I00/INDUSYNCON de Celis, M., Ruiz, J., Benitez-Dominguez, B. et al. Multi-omics framework to reveal the molecular determinants of fermentation performance in wine yeast populations. Microbiome 12, 203 (2024). https://doi.org/10.1186/s40168-024-01930-w Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ open access application/pdf BioMed Central