2026-06-08T00:03:50Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1100772025-03-18T12:53:48Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author González Guzmán, Luis Alfredo author Núñez Orjales, Ramón author Boquete Paris, Manuel author Pastor Vargas, Carlos author Bartolome, Borja author Carballada González, Francisco 2024-11-06T12:13:02Z 2024-11-06T12:13:02Z 2023 González Guzmán LA, Núñez Orjales R, Boquete Paris M, Pastor-Vargas C, Bartolome B, González FC. Clinical Characteristics and Sensitization Profile of Patients Allergic to Cow Epithelium. Int Arch Allergy Immunol. 2023;184(7):692-697. doi: 10.1159/000528855. Epub 2023 Mar 15. PMID: 36921582. 1018-24381423-0097 10.1159/000528855 https://hdl.handle.net/20.500.14352/110077 https://doi.org/10.1159/000528855 https://karger.com/iaa/article-abstract/184/7/692/836061/Clinical-Characteristics-and-Sensitization-Profile?redirectedFrom=fulltext Cow epithelium allergy (CEA) has been described in workers highly exposed to cattle, such as farmers and veterinarians, being a health problem in this population since it is their main livelihood. This study aimed to characterize the main clinical manifestations and define the sensitization profile of the cow epithelium-allergic population treated in our health area. Methods: This is a retrospective study including a total of 34 patients with a clinical diagnosis of CEA, confirmed by skin tests, bovine epithelium-specific IgE levels and allergen-specific conjunctival challenge test in some cases. They were distributed by age, sex, profession, clinical symptoms, specific IgE levels to other mammalian epithelia, pollens, mites, and foods. Immunoblotting was performed with extracts from cow dander, cow body fluids (urine and saliva), bull urine, and 17 sera from immunotherapy-untreated CEA patients. Results: The mean age of the patients was 44 years, with a higher incidence in cattle farmers. Rhinoconjunctivitis occurred in 100% of cases, with 35% having monosensitization to cow epithelium. Sera from most patients detected a 20-kDa IgE-binding band in cow dander, cow saliva, cow urine, and bull urine, corresponding to the major allergen Bos d 2 (bovine lipocalin). In 70% of the patients, a 25-kDa band was detected in cow and bull urine extracts, whose identification by mass spectrometry and investigation with protein databases led to the identification of a Bos taurus lipocalin (UniProt protein ID: A0A3Q1LGU7_BOVIN). Conclusion: CEA should be considered in patients exposed to cattle and as a cause of occupational disease. The IgE immunodetection revealed sensitization to a protein present in cow and bull urine (odorant-binding protein) not previously described. eng Attribution-NonCommercial-NoDerivatives 4.0 International Clinical characteristics and sensitization profile of patients allergic to cow epithelium journal article 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URL https://docta.ucm.es/bitstreams/6a597025-4e1e-4d66-af63-232ffe1fab01/download File MD5 95aaec23b247ecfad3084244a7a33e30 382369 application/pdf Clinical Characteristics and Sensitization Profile of Patients Allergic to Cow Epithelium.pdf URL https://docta.ucm.es/bitstreams/43486ef5-6215-435e-b1a5-502502665530/download File MD5 15d0230c5b1d216efde83458c6390522 22738 text/plain Clinical Characteristics and Sensitization Profile of Patients Allergic to Cow Epithelium.pdf.txt