2026-06-28T15:31:45Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1121092025-03-18T14:24:37Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Gamella Carballo, María author Ballesteros Redondo, María Isabel author Ruiz Valdepeñas Montiel, Víctor author Sánchiz, Africa author Cuadrado, Carmen author Pingarrón Carrazón, José Manuel author Linacero De La Fuente, M. Rosario author Campuzano Ruiz, Susana author 2024 This work reports the development and application of a disposable amperometric sensor built on magnetic microcarriers coupled to an Express PCR strategy to amplify a specific DNA fragment of the chloroplast trnH-psbA. The procedure involves the selective capture of a 68-mer synthetic target DNA (or unmodified PCR products) through sandwich hybridization with RNA capture probe-modified streptavidin MBs and RNA signaling probes, labeled using antibodies specific to the heteroduplexes and secondary antibodies tagged with horseradish peroxidase. Amperometric measurements were performed on screen-printed electrodes using the H2O2/hydroquinone system. Achieving a LOD of 3 pM for the synthetic target, it was possible to detect 2.5 pg of peanut DNA and around 10 mg kg−1 of peanut in binary mixtures (defatted peanut flours prepared in spelt wheat). However, the detectability decreased between 10 and 1000 times in processed samples depending on the treatment. The Express PCR-bioplatform was applied to the detection of peanut traces in foodstuff. Gamella M, Ballesteros MI, Ruiz-Valdepeñas Montiel V, Sánchiz A, Cuadrado C, Pingarrón JM, Linacero R, Campuzano S. Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA marker. Talanta 2024;277:126350. https://doi.org/10.1016/j.talanta.2024.126350. 0039-9140 10.1016/j.talanta.2024.126350 https://hdl.handle.net/20.500.14352/112109 1873-3573 https://doi.org/10.1016/j.talanta.2024.126350 https://www.sciencedirect.com/science/article/pii/S003991402400729X Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA marker