2026-06-27T15:15:42Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1121092025-03-18T14:24:37Zcom_20.500.14352_14col_20.500.14352_15

Gamella Carballo, María Ballesteros Redondo, María Isabel Ruiz Valdepeñas Montiel, Víctor Sánchiz, Africa Cuadrado, Carmen Pingarrón Carrazón, José Manuel Linacero De La Fuente, M. Rosario Campuzano Ruiz, Susana 2024-12-05T07:49:50Z 2024-12-05T07:49:50Z 2024 Gamella M, Ballesteros MI, Ruiz-Valdepeñas Montiel V, Sánchiz A, Cuadrado C, Pingarrón JM, Linacero R, Campuzano S. Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA marker. Talanta 2024;277:126350. https://doi.org/10.1016/j.talanta.2024.126350. 0039-9140 10.1016/j.talanta.2024.126350 https://hdl.handle.net/20.500.14352/112109 1873-3573 https://doi.org/10.1016/j.talanta.2024.126350 https://www.sciencedirect.com/science/article/pii/S003991402400729X This work reports the development and application of a disposable amperometric sensor built on magnetic microcarriers coupled to an Express PCR strategy to amplify a specific DNA fragment of the chloroplast trnH-psbA. The procedure involves the selective capture of a 68-mer synthetic target DNA (or unmodified PCR products) through sandwich hybridization with RNA capture probe-modified streptavidin MBs and RNA signaling probes, labeled using antibodies specific to the heteroduplexes and secondary antibodies tagged with horseradish peroxidase. Amperometric measurements were performed on screen-printed electrodes using the H2O2/hydroquinone system. Achieving a LOD of 3 pM for the synthetic target, it was possible to detect 2.5 pg of peanut DNA and around 10 mg kg−1 of peanut in binary mixtures (defatted peanut flours prepared in spelt wheat). However, the detectability decreased between 10 and 1000 times in processed samples depending on the treatment. The Express PCR-bioplatform was applied to the detection of peanut traces in foodstuff. eng http://creativecommons.org/licenses/by-nc/4.0/ open access Attribution-NonCommercial 4.0 International Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA marker journal article