2026-06-27T16:26:49Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1123592025-03-18T15:45:31Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Sevillano, Ester author Peña Vidal, Nuria author Cintas Izarra, Luis Miguel author Muñoz Atienza, Estefanía author Hernández Cruza, Pablo Elpidio author Borrero Del Pino, Juan 2024-12-10T16:24:18Z 2024-12-10T16:24:18Z 2024 Sevillano, E.; Lafuente, I.; Peña, N.; Cintas, L.M.; MuñozAtienza, E.; Hernández, P.E.; Borrero, J. Isolation, Genomics-Based and Biochemical Characterization of Bacteriocinogenic Bacteria and Their Bacteriocins, Sourced from the Gastrointestinal Tract of MeatProducing Pigs. Int. J. Mol. Sci. 2024, 25, 12210. https://doi.org/10.3390/ ijms252212210 1661-6596 10.3390/ijms252212210 https://hdl.handle.net/20.500.14352/112359 1422-0067 https://doi.org/10.3390/ijms252212210 Antimicrobial resistance (AMR) poses a significant challenge to animal production due to the widespread use of antibiotics. Therefore, there is an urgent need for alternative antimicrobial strategies to effectively manage bacterial infections, protect animal health, and reduce reliance on antibiotics. This study evaluated the use of emerging approaches and procedures for the isolation, identification, and characterization of bacteriocin-producing bacteria and their bacteriocins, sourced from the gastrointestinal tract (GIT) of meat-producing pigs. Out of 2056 isolates screened against Gram-positive and Gram-negative indicator strains, 20 of the most active antimicrobial isolates were subjected to whole genome sequencing (WGS) for the prediction of coding DNA sequences (CDS) and the identification of bacteriocin gene clusters (BGC) and their functions. The use of an in vitro cell-free protein synthesis (IV-CFPS) protocol and the design of an IV-CFPS coupled to a split-intein mediated ligation (IV-CFPS/SIML) procedure made possible the evaluation of the production and antimicrobial activity of described and putatively novel bacteriocins. A colony MALDI-TOF MS procedure assisted in the identification of class I, II, and III lanthipeptides. MALDI-TOF MS and a targeted proteomics, combined with a massive peptide analysis (LC-MS/MS) approach, has proven valuable for the identification and biochemical characterization of previously described and novel bacteriocins encoded by the isolated bacteriocin-producing strains. eng Attribution 4.0 International Isolation, Genomics-Based and Biochemical Characterization of Bacteriocinogenic Bacteria and Their Bacteriocins, Sourced from the Gastrointestinal Tract of Meat-Producing Pigs journal article 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